Protein Purification

Thermo Scientific™ T7 Cell-Free Expression Vectors

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Tissue

Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit

Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Thermo Scientific™ Pierce™ Avidin Agarose

Avidin biotin-binding protein immobilized onto beaded agarose resin for affinity purification methods involving biotinylated proteins and ligands.

R&D Systems Bestatin, CF

An aminopeptidase inhibitor and leukotrience A4 hydrolase inhibitor

Thermo Scientific™ MagnaBind™ Magnetic Beads and Magnets

Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.

R&D Systems Human Erythrocyte Lysing Kit

For the removal of erythrocytes (RBC) from splenocyte preparations.

Thermo Scientific™ Pierce™ p-Aminophenyl Phosphoryl Choline Agarose

Bind and purify C-reactive protein (CRP) using these agarose beads of immobilized p-aminophenyl phosphoryl choline (APC).

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Thermo Scientific™ Pierce™ Iminobiotin and Biotin Resins

Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.

Thermo Scientific™ Pierce™ Streptavidin Resins

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce™ DNase I

Cleave and degrade unwanted single- and double-stranded DNA

Thermo Scientific™ Pierce™ ConA and WGA Glycoprotein Isolation Kits

Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific Pierce™ qIP Protein Interaction Assay Reagents

Prepare optimized buffers for pull-down and reagent sets of TurboLuc (Tluc) assay buffer and coelenterazine substrate for luciferase quantitation with these reagents.

Thermo Scientific Expression Vectors for Pierce™ qIP Kits

Optimize cloning with this group of vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

R&D Systems Phosphoramidon, CF

A thermolysin-like metalloprotease inhibitor

Thermo Scientific™ Pierce™ GST (Glutathione S-Transferase)

Pure, lyophilized glutathione S-transferase (GST) protein from Schistosoma japonicum for use in recombinant fusion protein research methods and assays.

Thermo Scientific™ Pierce™ Protein G Magnetic Beads

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.

Thermo Scientific™ Pierce™ SDS-Out™ SDS Precipitation Reagent

Quickly remove SDS from small-volume (0.1 to 10mL) protein samples with this SDS precipitation kit.

Thermo Scientific™ Pierce™ Sodium Deoxycholate

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Thermo Scientific™ HiPPR Detergent Removal Resin and Kits

Efficiently remove detergents from samples with this high protein and peptide recovery detergent removal resin, especially for dilute protein samples.

Thermo Scientific™ Pierce™ Organelle Enrichment Kits

Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.

Thermo Scientific™ Pierce™ Glutathione Superflow Agarose

Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.

R&D Systems General Caspase Inhibitor Q-VD-OPh

Cell-permeable, irreversible inhibitor of caspase activity. Q-VD-OPh does not exhibit toxic effects at typical working concentrations.

Thermo Scientific™ Pierce™ Inclusion Body Solubilization Reagent

Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.

R&D Systems AEBSF, CF

A general, irreversible serine protease inhibitor

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