Protein Purification

Thermo Scientific™ Pierce™ DNase I

Cleave and degrade unwanted single- and double-stranded DNA

Thermo Scientific™ Pierce™ Iminobiotin and Biotin Resins

Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.

Thermo Scientific™ Pierce™ Organelle Enrichment Kits

Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.

Thermo Scientific™ Pierce™ Avidin Agarose

Avidin biotin-binding protein immobilized onto beaded agarose resin for affinity purification methods involving biotinylated proteins and ligands.

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit

Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.

Thermo Scientific™ SulfoLink™ Coupling Resins and Immobilization Kits

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

Thermo Scientific™ Pierce™ Monomeric Avidin Resins

Reversibly bind biotinylated proteins and other ligands with monomers of avidin covalently attached as individual subunits onto beaded resins.

Thermo Scientific™ Pierce™ HA Tag IP/Co-IP Kit

Anti-HA antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving HA-tagged fusion proteins.

Thermo Scientific™ T7 Cell-Free Expression Vectors

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Thermo Scientific Pierce™ Magnetic qIP Protein Interaction Kit, Tluc and HA Tags

Pull down (co-IP) and measure interactions between HA-tagged and Tluc-tagged protein pairs expressed in mammalian cells with this kit.

Thermo Scientific™ Pierce™ CHAPS

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific Pierce™ Magnetic qIP Protein Interaction Kit, Tluc and Myc Tags

Pull down (co-IP) and measure interactions between c-Myc-tagged and Tluc-tagged protein pairs expressed in mammalian cells.

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit

Easily isolate and culture highly viable mouse embryonic fibroblasts

Thermo Scientific™ Pierce™ Micrococcal Nuclease

Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Thermo Scientific™ Brij™-35 Detergent Solutions

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

Thermo Scientific™ Pierce™ Octyl beta-Glucoside

Nonionic detergent widely used for membrane protein solubilization that is dialyzable but susceptible to beta-Glucosidase digestion (unlike OTG).

Thermo Scientific Expression Vectors for Pierce™ qIP Kits

Optimize cloning with this group of vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Thermo Scientific Pierce™ Anti-c-Myc Agarose

Purify recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates with this immunoprecipitation resin.

Thermo Scientific™ AminoLink™ Coupling Resin and Immobilization Kit

Easily immobilize antibodies and other proteins for use in affinity purification

Thermo Scientific™ Pierce™ NHS-Activated Agarose

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

Thermo Scientific™ Pierce™ Streptavidin Resins

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

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