Protein Purification

Thermo Scientific™ Pierce™ n-Dodecyl-β-Maltoside

Water-soluble, non-denaturing and nonionic detergent especially useful for solubilizing hydrophobic membrane proteins to preserve their activity.

Thermo Scientific™ Brij™-35 Detergent Solutions

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

Thermo Scientific™ Pierce™ Sodium Deoxycholate

Water-soluble, ionic detergent used in cell lysis buffers (e.g., RIPA buffer) to disrupt protein interactions for liposome and membrane protein isolation.

Thermo Scientific™ Pierce™ CHAPS

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ Tween-20 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ AminoLink™ Coupling Resin and Immobilization Kit

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

Thermo Scientific™ Pierce™ Streptavidin Resins

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific Pierce™ qIP Protein Interaction Assay Reagents

Prepare optimized buffers for pull-down and reagent sets of TurboLuc (Tluc) assay buffer and coelenterazine substrate for luciferase quantitation with these reagents.

Thermo Scientific™ Pierce™ Glutathione Agarose Resin and Kits

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce™ Octyl beta-Glucoside

Nonionic detergent widely used for membrane protein solubilization that is dialyzable but susceptible to beta-Glucosidase digestion (unlike OTG).

Thermo Scientific™ Low Protein Binding Collection Tubes, 2.0 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (2.0 mL).

Thermo Scientific™ HisPur™ Cobalt Resin

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.

Thermo Scientific™ Pierce™ GST (Glutathione S-Transferase)

Pure, lyophilized glutathione S-transferase (GST) protein from Schistosoma japonicum for use in recombinant fusion protein research methods and assays.

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Thermo Scientific™ NP-40 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Thermo Scientific™ AminoLink™ Plus Coupling Resin and Immobilization Kits

Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.

Thermo Scientific™ Pierce™ Coated Plate IP Kits

Perform immunoprecipitation assays without resins, beads, centrifugation or magnets using coated streptavidin or Protein A/G 96-well microplates.

Thermo Scientific™ UltraLink™ Biosupport

Covalently immobilize proteins and other primary amine biomolecules for affinity purification with this durable, azlactone-activated polyacrylamide resin.

Thermo Scientific™ T7 Cell-Free Expression Vectors

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Thermo Scientific™ Pierce™ Avidin Agarose

Avidin biotin-binding protein immobilized onto beaded agarose resin for affinity purification methods involving biotinylated proteins and ligands.

Thermo Scientific™ Pierce™ GST Agarose

Isolate or remove cross-reacting GST-specific serum or other antibody solutions with immobilized glutathione S-transferase agarose.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific™ Pierce™ Classic IP Kit

Perform traditional immunoprecipitation assays with ease using high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers.

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

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