Protein Purification

Thermo Scientific™ Pierce™ CHAPS

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific Pierce™ qIP Protein Interaction Assay Reagents

Prepare optimized buffers for pull-down and reagent sets of TurboLuc (Tluc) assay buffer and coelenterazine substrate for luciferase quantitation with these reagents.

Thermo Scientific™ T7 Cell-Free Expression Vectors

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Pierce™ Coated Plate IP Kits

Perform immunoprecipitation assays without resins, beads, centrifugation or magnets using coated streptavidin or Protein A/G 96-well microplates.

Thermo Scientific Expression Vectors for Pierce™ qIP Kits

Optimize cloning with this group of vectors, which includes epitope-tagged (HA and c-Myc) and TurboLuc-tagged (Tluc) multiple cloning site mammalian expression vectors.

Thermo Scientific™ Pierce™ Octyl beta-Glucoside

Nonionic detergent widely used for membrane protein solubilization that is dialyzable but susceptible to beta-Glucosidase digestion (unlike OTG).

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins

Acrylamide and agarose beads and columns of specially-modified, pH-stabilized avidin; the low-cost alternative to streptavidin biotin-binding supports.

Thermo Scientific™ UltraLink™ Biosupport

Covalently immobilize proteins and other primary amine biomolecules for affinity purification with this durable, azlactone-activated polyacrylamide resin.

Thermo Scientific™ Pierce™ Protein AG Magnetic Beads

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

Thermo Scientific™ Pierce™ Protein A Magnetic Beads

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.

Thermo Scientific™ Pierce™ Streptavidin Resins

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce™ Phosphatase Inhibitor Tablets

Protect serine, threonine and tyrosine phosphorylation in your sample with these broad-spectrum phosphatase inhibitor tablets.

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ 1-Step Human Coupled In Vitro Protein Expression Kits

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction using these cell-free kits.

Thermo Scientific™ Pierce™ Mitochondria Isolation Kit for Cultured Cells

Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ Pierce™ Sodium Dodecyl Sulfate (SDS) C12

Highly purified form of SDS especially suited for protein experiments requiring tightly controlled solubility parameters.

Thermo Scientific™ anti-HA Epitope Tag Clone: 2-2.2.14

HA Epitope Tag Monoclonal antibody 26181 from Thermo Scientific™ specifically detects HA Epitope Tag in Tag samples, and it is validated for Immunoprecipitation

Thermo Scientific™ MagnaBind™ Magnetic Beads and Magnets

Enable benchtop magnetic affinity purification with these ligand-coated (streptavidin, Protein A and G, GAM IgG, GAR IgG) magnetic particles and magnet separators.

Thermo Scientific 1-Step Human High-Yield In Vitro Expression Kits

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

Thermo Scientific™ Pierce™ IgG Plus Orientation Kits

Bind and covalently immobilize antibodies to create a reusable affinity purification column with high binding capacity with Protein A or G agarose columns and DSS crosslinker.

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Thermo Scientific™ Pierce™ Protein G Magnetic Beads

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.

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