Protein Isolation and Purification

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Triton™ X-100 (Electrophoresis), Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO

GE Healthcare HiScale™ Chromatography Columns Available on GSA/VA Contract for Federal Government customers only.

Withstand pressures of up to 20 bar (290 psi) and high flow rates. GE Healthcare Life Sciences™ HiScale™ Chromatography Columns are designed to be compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™.

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

GE Healthcare PreScission™ Protease Available on GSA/VA Contract for Federal Government customers only.

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. GE Healthcare Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

GE Healthcare XK Chromatography Columns (Empty) Available on GSA/VA Contract for Federal Government customers only.

Ensure trouble-free operation for low to medium pressure. GE Healthcare XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.

GE Healthcare Mono Q PC, Columns Prepacked with MonoBeads™ Available on GSA/VA Contract for Federal Government customers only.

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.

Promega™ Nano-Glo™ Luciferase Assay System

Provides a single-addition reagent that generates a glow-type signal in the presence of NanoLuc™ luciferase

GE Healthcare PD MultiTrap™ Plates Available on GSA/VA Contract for Federal Government customers only.

Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with high reproducibility from well to well and from plate to plate

GE Healthcare Superose™ 6 Increase Columns Available on GSA/VA Contract for Federal Government customers only.

High-resolution size-exclusion chromatography for studying large proteins and complexes.   GE Healthcare Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Mono S PC, Columns Prepacked with MonoBeads™ Available on GSA/VA Contract for Federal Government customers only.

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.

GE Healthcare PD MiniTrap™ G-25 Sample Preparation Columns Available on GSA/VA Contract for Federal Government customers only.

Use for cleanup of biomolecules with a molecular weight  >5000 Mr in sample volumes up to 0.5mL.  GE Healthcare Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

EMD Millipore™ D-Tube Dialyzer Maxi, MWCO 3.5 kDa Available on GSA/VA Contract for Federal Government customers only.

Used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels

GE Healthcare Tricorn Columns Available on GSA/VA Contract for Federal Government customers only.

Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents Available on GSA/VA Contract for Federal Government customers only.

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

GE Healthcare PD MidiTrap™ G-25 Sample Preparation Columns Available on GSA/VA Contract for Federal Government customers only.

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL.  GE Healthcare Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Sartorius™ Sartoclear Dynamics™: Lab V

For simultaneous clarification and sterile filtration of 50 mL to 1 L mammalian cell cultures in one step without centrifugation.

GE Healthcare LabMate™ PD-10 Buffer Reservoirs Available on GSA/VA Contract for Federal Government customers only.

Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. GE Healthcare Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.

GE Healthcare Ni Sepharose™ 6 Fast Flow Available on GSA/VA Contract for Federal Government customers only.

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

GE Healthcare HiTrap™ Protein L Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

GE Healthcare StrepTrap™ HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify Strep(II)-tagged recombinant proteins by affinity chromatography. GE Healthcare Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.

GE Healthcare Thrombin Protease Available on GSA/VA Contract for Federal Government customers only.

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

GE Healthcare StrepTactin Sepharose High Performance Available on GSA/VA Contract for Federal Government customers only.

Preserve target protein activity during purification. GE Healthcare StrepTactin Sepharose High Performance is a robust and stable Sepharose-based medium that results in high resolution and reproducible purifications.

GE Healthcare HiTrap™ Chelating HP Columns Available on GSA/VA Contract for Federal Government customers only.

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

EMD Millipore™ PureProteome™ Albumin Magnetic Beads Available on GSA/VA Contract for Federal Government customers only.

Faster, easier serum depletion with greater insight into your sample

EMD Millipore™ D-Tube96™ Dialyzer, 12-14 kDa Available on GSA/VA Contract for Federal Government customers only.

Convenient and simultaneous dialysis of 96 samples in high throughput manner

GE Healthcare Ni Sepharose™ excel Available on GSA/VA Contract for Federal Government customers only.

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

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