Protein Isolation and Purification

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

Chemical Name or Material: Sodium Dodecyl Sulfate CAS: 151-21-3 CAS: 7647-14-5 CAS: 7757-82-6 Purity Grade: Electrophoresis Grade Purity Grade Notes: DNase- and RNase-Free Physical Form: Solid Assay: ≥99% (≥98% C12-OH) Assay Percent Range: (as Fatty Alcohol Sulfate) >=99% Assay Percent Notes: (as Fatty Alcohol Sulfate) >=99% Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na Synonym: Sodium Lauryl Sulfate, SDS

Triton™ X-100 (Electrophoresis), Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: Triton™ X-100 CAS: 9002-93-1 Purity Grade: Electrophoresis Grade Physical Form: Liquid Linear Formula: 4-(C8H17) C6H4 (OCH2CH2)n OH (n∽10) Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether

GE Healthcare PreScission™ Protease Available on GSA/VA Contract for Federal Government customers only.

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. GE Healthcare Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

GE Healthcare Superose™ 6 Increase Columns Available on GSA/VA Contract for Federal Government customers only.

High-resolution size-exclusion chromatography for studying large proteins and complexes.   GE Healthcare Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: Sodium Dodecyl Sulfate Name Note: 20% Solution CAS: 151-21-3 CAS: 7732-18-5 Purity Grade: Electrophoresis/Molecular Biology-Grade Purity Grade Notes: DNase-, RNase- and Protease-Free Physical Form: Liquid Assay: ≥99% Assay Percent Range: (as Fatty Alcohol Sulfate): ≥99% Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na Synonym: Sodium lauryl sulfate, SDS

GE Healthcare Mono Q PC, Columns Prepacked with MonoBeads™ Available on GSA/VA Contract for Federal Government customers only.

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

GE Healthcare HiScale™ Chromatography Columns Available on GSA/VA Contract for Federal Government customers only.

Withstand pressures of up to 20 bar (290 psi) and high flow rates. GE Healthcare Life Sciences™ HiScale™ Chromatography Columns are designed to be compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™.

GE Healthcare PD MiniTrap™ G-25 Sample Preparation Columns Available on GSA/VA Contract for Federal Government customers only.

Use for cleanup of biomolecules with a molecular weight  >5000 Mr in sample volumes up to 0.5mL.  GE Healthcare Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promega™ Nano-Glo™ Luciferase Assay System

Provides a single-addition reagent that generates a glow-type signal in the presence of NanoLuc™ luciferase

GE Healthcare XK Chromatography Columns (Empty) Available on GSA/VA Contract for Federal Government customers only.

Ensure trouble-free operation for low to medium pressure. GE Healthcare XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.

GE Healthcare PD MultiTrap™ Plates Available on GSA/VA Contract for Federal Government customers only.

Convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr) with high reproducibility from well to well and from plate to plate

GE Healthcare Ni Sepharose™ 6 Fast Flow Available on GSA/VA Contract for Federal Government customers only.

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

GE Healthcare PD MidiTrap™ G-25 Sample Preparation Columns Available on GSA/VA Contract for Federal Government customers only.

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL.  GE Healthcare Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

CHAPS (White Crystalline Powder), Fisher BioReagents™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: CHAPS CAS: 75621-03-3 Physical Form: Solid Assay Percent Range: ≥98 % Molecular Formula: C32H58N2O7S Synonym: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate

GE Healthcare Tricorn Columns Available on GSA/VA Contract for Federal Government customers only.

Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: Sodium Dodecyl Sulfate (SDS), Micropellets CAS: 151-21-3 Purity Grade: Molecular Biology Grade Physical Form: Solid Assay: ≥98.0% as C12 alcohol Assay Percent Range: >99%

GE Healthcare HiTrap™ Chelating HP Columns Available on GSA/VA Contract for Federal Government customers only.

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

GE Healthcare Whatman™ 903 Neonatal Screening Cards Available on GSA/VA Contract for Federal Government customers only.

903 sample collection card with four sample spots.

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

Chemical Name or Material: Sodium Dodecyl Sulfate Name Note: 10% Solution CAS: 151-21-3 CAS: 7732-18-5 Purity Grade: Electrophoresis Grade Assay Percent Range: ≥99 % Molecular Formula: C12H25NaO4S Linear Formula: CH3(CH2)10CH2OSO3Na

GE Healthcare LabMate™ PD-10 Buffer Reservoirs Available on GSA/VA Contract for Federal Government customers only.

Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. GE Healthcare Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.

GE Healthcare Thrombin Protease Available on GSA/VA Contract for Federal Government customers only.

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

GE Healthcare StrepTrap™ HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify Strep(II)-tagged recombinant proteins by affinity chromatography. GE Healthcare Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.

GE Healthcare Ni Sepharose™ excel Available on GSA/VA Contract for Federal Government customers only.

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation of DYKDDDDK-tagged proteins

GE Healthcare HiTrap™ Protein L Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

GE Healthcare StrepTactin™ Sepharose™ High Performance Medium Available on GSA/VA Contract for Federal Government customers only.

Mild purification conditions preserve antigenicity and function. GE Healthcare StrepTactin Sepharose High Performance media is used for robust, high resolution purification of Strep II-tagged proteins.

EMD Millipore™ D-Tube™ Dialyzer Mega, 20mL, MWCO 6-8 kDa Available on GSA/VA Contract for Federal Government customers only.

Used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels

D-Tube Dialyzer Mega, 15mL, MWCO 3.5 kDa Available on GSA/VA Contract for Federal Government customers only.

Used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels

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