Bioassay Systems

Bioassay Systems Phosphatase Inhibitor. Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Uses: to preserve protein phosphorylation state in cells and lysates.

Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays. Key Features: Ready-to-use phosphatase inhibitor. Fully activated for maximum inhibition. Kit size: 1 mL 100 mM. Sodium Orthovanadate (CAS Number 13721-39-6, Na3VO4) is a general inhibitor for protein phosphotyrosyl phosphatases (PTPs). Inhibition of vanadate is competitive and can be reversed by addition of EDTA or by dilution. Vanadate is commonly used to preserve the protein tyrosyl phosphorylation state in cells and lysates. BioAssay Systems Vanadate reagent is activated for maximum inhibition. Suggested Working Concentration: 1-10 mM

Bioassay Systems Polyvinylpyrrolidone (PVPP) used for preparation of wine samples for use with the QuantiQuik™ Histamine Quick Test Strips.

Polyvinylpyrrolidone (PVPP) used for preparation of wine samples for use with the QuantiQuik™ Histamine Quick Test Strips.

Bioassay Systems EnzyChrom™ Urea Assay Kit III. For the quantitative enzymatic determination of urea

For the quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 50 to 1000 μM urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm; Samples: Urea in biological samples (e.g. plasma, serum, urine, bronchoalveolar lavage (BAL)) and food/beverage samples (e.g. milk). Size: 100 tests; Detection Limit: 50 µM.

Bioassay Systems QuantiChrom™ Arginase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of arginase inhibitors.

For evaluation and high-throughput screen (HTS) of arginase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 2 hours and no 37°C heater is needed. Method: OD430nm. Samples: Arginase. Species: All. Procedure: Assay takes 1 hour 45 min. Kit size: 100 tests.

Bioassay Systems EnzyChrom™ Phospholipase D Assay Kit. For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism. Key Features: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.04 U/L.

Bioassay Systems EnzyChrom™ Xanthine Oxidase Assay Kit. For quantitative determination of xanthine oxidase enzyme activity.

For quantitative determination of xanthine oxidase enzyme activity. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Method: OD570nm, FL530/585nm; Samples: Cell lysate, serum, and other biological samples; Size: 100 tests.

Bioassay Systems QuantiFluo™ Acid Phosphatase Assay Kit. Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.

Bioassay Systems QuantiQuik™ Malic Acid Quick Test Strips. Direct determination of malic acid concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of malic acid concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-500 mg/L (undiluted) malic acid. Convenient. No expensive lab equipment needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems EnzyChrom™ Acetylcholine Assay Kit. For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.

For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 10 to 200 µM, fluorimetric assay 0.4 to 10 µM acetylcholine. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 10, 0.4 µM.

Bioassay Systems QUANTICHROM PLASMIN ASSAY KIT

NC2902721 QUANTICHROM PLASMIN ASSAY KIT

Bioassay Systems QuantiChrom™ Whole Blood Hemoglobin Kit. For quantitative determination of hemoglobin (Hb) in whole blood.

For quantitative determination of hemoglobin (Hb) in whole blood. Key Features: Safety. Non-hazardous. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvette. High sensitivity. As low as 5 µL sample is used. Ideal when samples are limited such as mice blood samples. Method: OD570nm. Samples: Whole blood samples. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.2 g/dL.

Bioassay Systems EnzyFluo™ L-Lactate Assay Kit. For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.

For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM L-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM.

Bioassay Systems EnzyLight™ ADP/ATP Ratio Assay Kit. For quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells.

For quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells and screen for modulators. Key Features: Safe. Non-radioactive assay. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.

Bioassay Systems QuantiChrom™ Formaldehyde Assay Kit. Direct determination of formaldehyde concentrations in biological, food and environmental samples

For quantitative and direct determination of formaldehyde concentrations in biological, food and environmental samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 1.5 µM (45 ppb) formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL370/470nm. Samples: Biological, food, beverage, environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 1.5 µM (45 ppb).