BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy the ever increasing demands of the life sciences industry.
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For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
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For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators. Key Features: Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at room temperature. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Acetylcholinesterase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.
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For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Key Features: Sensitive and accurate. Detection limit of 50 µM, linearity up to 2.5 mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.
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For quantitative determination of invertase/sucrase enzyme activity. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological, environment (soil) etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.007 U/L.
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A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot. Key Features: Highly sensitive. Detects as low as 7.6x10-8 U/well, or 7.7 pg/well horseradish peroxidase (HRP) in 96-well plate assays. Detects as low as 0.1 ppb Gliadin in ELISA. Improved stability and assay robustness. Method: OD450nm. Samples: peroxidases and HRP in ELISA. Species: All. Procedure: Assay takes Approx. 30 min. Kit size: 200 Tests. Detection limit: 76 nU/well, or 7.7 pg/well. HORSERADISH PEROXIDASE (HRP) is an enzyme commonly used when conjugated to analytes of interest. When combined with hydrogen peroxide and a dye, HRP can be used to colorimetrically quantify the analyte.
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For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism. Key Features: Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H2O2 in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD585nm. Samples: Serum, citrate-plasma, urine, cell lysate, culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 µM.
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For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl3 reagent, the time required for reduction of nitrate (NO3-) to nitrite (NO2-) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.
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For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed. Method: OD565nm. Samples: Glucose-6-phosphate dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.
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For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism. Key Features: Sensitive and accurate. Linear detection range: 2 to 400 µM aspartate for colorimetric assays and 1 to 50 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue, culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 1 µM.
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For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.
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For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.
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For quantitative determination of chromium Cr(VI). Key Features: Sensitive and accurate. Linear detection range of 20 - 2000 µg/L Chromium. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples. Species: All. Procedure: Assay takes 20 min. Kit size: 250 tests. Detection limit: 20 µg/L (0.38 µM, 0.02 ppm).
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For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM.
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For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Linear detection range: 7 to 400 µM oxaloacetate for colorimetric assays and 1 to 40 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue and culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 1 µM.
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For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators. Key Features: High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 3 hours if assay performed at 37°C. Method: OD540nm. Samples: Nitric Oxide Synthase. Species: All. Procedure: Assay takes 2-3 hours. Kit size: 100 tests.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More