Bioassay Systems

Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems QuantiChrom&trad; Acetylcholinesterase Inhibitor Assay Kit. For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators.

For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators. Key Features: Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at room temperature. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Acetylcholinesterase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

Bioassay Systems EnzyChrom™ Glutamate Assay Kit. For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism.

For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Key Features: Sensitive and accurate. Detection limit of 50 µM, linearity up to 2.5 mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.

Bioassay Systems EnzyChrom™ Invertase Assay Kit. For quantitative determination of invertase/sucrase activity.

For quantitative determination of invertase/sucrase enzyme activity. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological, environment (soil) etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.007 U/L.

Bioassay Systems QuantiChrom™ HRP Detection Reagent. A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot.

A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot. Key Features: Highly sensitive. Detects as low as 7.6x10-8 U/well, or 7.7 pg/well horseradish peroxidase (HRP) in 96-well plate assays. Detects as low as 0.1 ppb Gliadin in ELISA. Improved stability and assay robustness. Method: OD450nm. Samples: peroxidases and HRP in ELISA. Species: All. Procedure: Assay takes Approx. 30 min. Kit size: 200 Tests. Detection limit: 76 nU/well, or 7.7 pg/well.
HORSERADISH PEROXIDASE (HRP) is an enzyme commonly used when conjugated to analytes of interest. When combined with hydrogen peroxide and a dye, HRP can be used to colorimetrically quantify the analyte.

Bioassay Systems QuantiChrom™ Peroxide Assay Kit. For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism.

For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism. Key Features: Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H₂O₂ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD585nm. Samples: Serum, citrate-plasma, urine, cell lysate, culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 µM.

Bioassay Systems QuantiChrom™ Nitric Oxide Assay Kit. For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism.

For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl₃ reagent, the time required for reduction of nitrate (NO₃^-) to nitrite (NO₂^-) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.

Bioassay Systems QuantiChrom™ G6PDH Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed. Method: OD565nm. Samples: Glucose-6-phosphate dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

Bioassay Systems EnzyChrom™ Aspartate Assay Kit. For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism. Key Features: Sensitive and accurate. Linear detection range: 2 to 400 µM aspartate for colorimetric assays and 1 to 50 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue, culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 1 µM.

Bioassay Systems EnzyLight™ Cytotoxicity Assay Kit. For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxicity and high-throughput screen for anticancer agents.

For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.

Bioassay Systems EnzyFluo™ AMPK Phosphorylation Assay Kit. For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.

For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.

Bioassay Systems QuantiChrom&trade Chromium Assay Kit. For quantitative determination of chromium Cr(VI).

For quantitative determination of chromium Cr(VI). Key Features: Sensitive and accurate. Linear detection range of 20 - 2000 µg/L Chromium. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples. Species: All. Procedure: Assay takes 20 min. Kit size: 250 tests. Detection limit: 20 µg/L (0.38 µM, 0.02 ppm).

Bioassay Systems EnzyFluo™ D-Lactate Assay Kit. For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 1 µM.

Bioassay Systems EnzyChrom™ Oxaloacetate Assay Kit. For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism.

For quantitative determination of oxaloacetate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Linear detection range: 7 to 400 µM oxaloacetate for colorimetric assays and 1 to 40 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue and culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 1 µM.

Bioassay Systems EnzyChrom™ Nitric Oxide Synthase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of nitric oxide synthase inhibitors.

For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators. Key Features: High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 3 hours if assay performed at 37°C. Method: OD540nm. Samples: Nitric Oxide Synthase. Species: All. Procedure: Assay takes 2-3 hours. Kit size: 100 tests.