Bioassay Systems

Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems EnzyChrom™ Aspartate Assay Kit. For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism. Key Features: Sensitive and accurate. Linear detection range: 2 to 400 µM aspartate for colorimetric assays and 1 to 50 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue, culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 1 µM.

Bioassay Systems QuantiChrom™ G6PDH Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed. Method: OD565nm. Samples: Glucose-6-phosphate dehydrogenase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.

Bioassay Systems EnzyFluo™ Isocitrate Assay Kit. For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585 nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.6 µM.

Bioassay Systems EnzyLight™ Cytotoxicity Assay Kit. For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxicity and high-throughput screen for anticancer agents.

For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.

Bioassay Systems QuantiChrom™ Glutamate Dehydrogenase Assay Kit. For quantitative determination of glutamate dehydrogenase activity.

For quantitative determination of glutamate dehydrogenase activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell, tissue, agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 U/L.

Bioassay Systems EnzyChrom™ L-Alanine Assay Kit. For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism.

For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 200 µM alanine for colorimetric assays and 0.4 to 20 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.4 µM.

Bioassay Systems EnzyChrom™ Ethanol Assay Kit. For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism.

For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism. Key Features: Sensitive and accurate. Detection limit 0.0008 vol % (140 µM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay. Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm (Enzymatic). Samples: Serum, plasma, urine, saliva samples etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.0008%.

Bioassay Systems EnzyChrom™ Choline Assay Kit. For quantitative determination of choline and evaluation of drug effects on choline metabolism.

For quantitative determination of choline and evaluation of drug effects on choline metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, cells etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 µM.

Bioassay Systems EnzyChrom™ Creatine Assay Kit. For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.

For quantitative determination of creatine and evaluation of drug effects on creatine metabolism. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Linear detection range 4 to 1000 µM (colorimetric) or 0.5 to 50 µM (fluorimetric). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes. Method: OD570nm, or FL530/590nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 4 µM.

Bioassay Systems QuantiChrom™ Pectinase Assay Kit. For quantitative determination of pectinase activity determination in biological samples.

For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.

Bioassay Systems QuantiChrom™ Nitric Oxide Assay Kit. For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism.

For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl₃ reagent, the time required for reduction of nitrate (NO₃^-) to nitrite (NO₂^-) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.

Bioassay Systems SuperLight™ Luciferase Reporter Gene Assay Kit (500 tests). Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.

Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens. Key Features: High sensitivity and wide detection range. The emitted light is linear over seven orders of magnitude. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems. Fast and convenient. Homogeneous "mix-and-measure". The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection. Robust and amenable to HTS. Z factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 2 min. Kit size: 1000 tests. Detection limit: 2 fg luciferase.

Bioassay Systems EnzyChrom™ Glutamate Assay Kit. For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism.

For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. Key Features: Sensitive and accurate. Detection limit of 50 µM, linearity up to 2.5 mM glutamate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.

Bioassay Systems EnzyFluo™ AMPK Phosphorylation Assay Kit. For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.

For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.