Bioassay Systems

Bioassay Systems Phosphatase Inhibitor. Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Uses: to preserve protein phosphorylation state in cells and lysates.

Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays. Key Features: Ready-to-use phosphatase inhibitor. Fully activated for maximum inhibition. Kit size: 1 mL 100 mM. Sodium Orthovanadate (CAS Number 13721-39-6, Na3VO4) is a general inhibitor for protein phosphotyrosyl phosphatases (PTPs). Inhibition of vanadate is competitive and can be reversed by addition of EDTA or by dilution. Vanadate is commonly used to preserve the protein tyrosyl phosphorylation state in cells and lysates. BioAssay Systems Vanadate reagent is activated for maximum inhibition. Suggested Working Concentration: 1-10 mM

Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems QUANTIFLUO ADENOSINE DEAMINASE

NC3233017 QUANTIFLUO ADENOSINE DEAMINASE

Bioassay Systems EnzyChrom™ Malate Dehydrogenase Assay Kit. For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.

For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 65 U/L for 20 min reaction at 37°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

Bioassay Systems QuantiChrom™ Biotin Assay Kit. Quantitative determination of biotin in food, cosmetic, and biotinylated proteins.

For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.

Bioassay Systems QuantiChrom™ Indole Assay Kit. Indole determination in biological samples.

For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µM.

Bioassay Systems QuantiChrom™ Arginase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of arginase inhibitors.

For evaluation and high-throughput screen (HTS) of arginase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 2 hours and no 37°C heater is needed. Method: OD430nm. Samples: Arginase. Species: All. Procedure: Assay takes 1 hour 45 min. Kit size: 100 tests.

Bioassay Systems EnzyChrom™ Fumarate Assay Kit. For quantitative determination of fumarate and drug effects on its metabolism.

For quantitative determination of fumarate and drug effects on its metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.005 to 2 mM fumarate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 5 µM.

Bioassay Systems EnzyChrom™ Monoamine Oxidase Assay Kit. For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen.

For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: MAO enzyme preparations. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

Bioassay Systems EnzyChrom™ Monoamine Oxidase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of monoamine oxidase inhibitors.

For evaluation and high-throughput screen (HTS) of monoamine oxidase modulators. Key Features: Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Monoamine Oxidase. Species: All. Procedure: Assay takes 35 min. Kit size: 100 tests.

Bioassay Systems QuantiFluo™ Acid Phosphatase Assay Kit. Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.

Bioassay Systems EnzyChrom™ Xanthine Oxidase Assay Kit. For quantitative determination of xanthine oxidase enzyme activity.

For quantitative determination of xanthine oxidase enzyme activity. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Method: OD570nm, FL530/585nm; Samples: Cell lysate, serum, and other biological samples; Size: 100 tests.

Bioassay Systems EnzyChrom™ Acetaldehyde Assay Kit. For the quantitative determination of acetaldehyde in biological and food/beverage samples

For the quantitative determination of acetaldehyde in biological and food/beverage samples. Method: OD565nm; Samples: Biological, food and beverage samples; Size: 100 tests; Detection Limit: 2 µM. Key Features: Fast and sensitive. Linear detection range (20µL sample): 2µM to 2 mM acetaldehyde in 96-well plate assay. High-throughput. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. Convenient. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Bioassay Systems EnzyChrom™ Maltose Assay Kit.Quantitative determination of maltose in various biological samples such as serum, urine, food and beverages, etc.

For quantitative determination of maltose and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for flourimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, urine, food and beverages, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 2 µM.

Bioassay Systems QuantiChrom™ Β-N-Acetylglucosaminidase Assay Kit.Β-N-Acetylglucosaminidase activity determination in biological samples.

For quantitatve determination of β-N-Acetylglucosaminidase activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 50 U/L for a 30 minute reaction at 37°C. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD405nm. Samples: Urine, serum, plasma, cell lysate, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.2 U/L.