Bioassay Systems
BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy the ever increasing demands of the life sciences industry.
Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.
For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems QuantiChrom™ Protein Creatinine Ratio Assay Kit. For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR).
For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR). Key Features: Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 - 150 mg/dL Creatinine. Fast and convenient. No sample pre-trea™ent is needed. Simple 10- minute "add-incubate-read" procedure. High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day. Method: OD600 and OD530nm. Samples: Urine samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 1 mg/dL Protein and 1 mg/dL Creatinine.
Bioassay Systems QuantiChrom™ Arginase Assay Kit. For quantitative determination of arginase activity and screen for its inhibitors.
For quantitative determination of arginase activity and screen for its inhibitors. Key Features: Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD430nm. Samples: Enzyme preparations, serum, plasma, tissue culture etc. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: 1 U/L (0.3 U/L for 2 hr reaction).
Bioassay Systems QuantiChrom&trad; Acetylcholinesterase Inhibitor Assay Kit. For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators.
For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators. Key Features: Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at room temperature. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Acetylcholinesterase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.
Bioassay Systems QuantiChrom™ Sorbitol Dehydrogenase Assay Kit. For quantitative determination of sorbitol dehydrogenase enzyme activity.
For quantitative determination of sorbitol dehydrogenase enzyme activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples e.g. plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.1 U/L.
Bioassay Systems QuantiChrom™ Peroxidase Assay Kit. For quantitative determination of peroxidase enzyme activity.
For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L.
PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
ROOR + electron donor (2 e-) + 2H+ → ROH + R'OH.
Bioassay Systems EnzyChrom™ Glucose Oxidase Assay Kit. For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell/tissue lysate, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 (OD), 0.002U/L (FL).
Bioassay Systems QuantiChrom™ Detergent Assay Kit. For quantitative determination of detergents in e.g. water and soil samples.
For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
Bioassay Systems QuantiChrom™ Indole Assay Kit. Indole determination in biological samples.
For quantitative determination of indole and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 100µL sample. Linear detection range from 3 to 100 µM indole in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately. Method: OD565nm. Samples: Bacterial growth media and other biological samples. Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 3 µM.
Bioassay Systems EnzyChrom™ Malate Assay Kit. For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism.
For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Malate in food, juice, beverage and other agricultural products. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.02 mM.
Bioassay Systems EnzyChrom™ Phospholipase D Assay Kit. For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism. Key Features: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L. Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.04 U/L.
Bioassay Systems QuantiQuik™ Glucose Quick Test Strips. Direct determination of glucose concentrations in food and beverage samples as well as biological (e.g. urine, blood, etc) samples.
For rapid, direct determination of glucose concentrations in food and beverage samples as well as biological (e.g. urine, blood, etc.) samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-720 mg/L (undiluted) Glucose. Convenient. No expensive lab equipment needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Wine, beer, juice, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems EnzyFluo™ Isocitrate Assay Kit. For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.
For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585 nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.6 µM.
Bioassay Systems EnzyChrom™ Glucose-6-Phosphate Assay Kit. For quantitative determination of glucose-6-phosphate in biological samples.
For quantitative determination of glucose-6-phosphate in biological samples. Key Features: Fast and sensitive. Linear detection range: 10 to 1000 µM G6P. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD460nm. Samples: Plasma, serum, tissue and culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 10 µM.
Bioassay Systems QuantiFluo™ Alkaline Phosphatase Assay Kit. For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate.
For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Detection limit of 0.02 U/L (20 min reaction). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: FL360/450nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 U/L.