Bioassay Systems
BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy the ever increasing demands of the life sciences industry.
Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.
For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems Phosphatase Inhibitor. Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Uses: to preserve protein phosphorylation state in cells and lysates.
Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays. Key Features: Ready-to-use phosphatase inhibitor. Fully activated for maximum inhibition. Kit size: 1 mL 100 mM. Sodium Orthovanadate (CAS Number 13721-39-6, Na3VO4) is a general inhibitor for protein phosphotyrosyl phosphatases (PTPs). Inhibition of vanadate is competitive and can be reversed by addition of EDTA or by dilution. Vanadate is commonly used to preserve the protein tyrosyl phosphorylation state in cells and lysates. BioAssay Systems Vanadate reagent is activated for maximum inhibition. Suggested Working Concentration: 1-10 mM
Bioassay Systems QuantiQuik™ Histamine Quick Test Strips. For rapid, direct determination of Histamine concentrations in fish, wine, and other samples.
For rapid, direct determination of Histamine concentrations in fish, wine, and other samples. Key Features: Fast and sensitive. Use of 500 mg of fish or 250 µL of sample. Semi-quantitative measurement between 0-200 ppm (undiluted) Histamine. Convenient. No expensive lab equipment needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Seafood, wine, juice, milk and dairy products, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems QuantiChrom&trad; Acetylcholinesterase Inhibitor Assay Kit. For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators.
For evaluation and high-throughput screen (HTS) of acetylcholinesterase modulators. Key Features: Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at room temperature. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD412nm. Samples: Acetylcholinesterase. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests.
Bioassay Systems QuantiChrom™ Peroxidase Assay Kit. For quantitative determination of peroxidase enzyme activity.
For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L.
PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
ROOR + electron donor (2 e-) + 2H+ → ROH + R'OH.
Bioassay Systems QuantiChrom™ Sorbitol Dehydrogenase Assay Kit. For quantitative determination of sorbitol dehydrogenase enzyme activity.
For quantitative determination of sorbitol dehydrogenase enzyme activity. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples e.g. plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.1 U/L.
Bioassay Systems QuantiChrom™ Detergent Assay Kit. For quantitative determination of detergents in e.g. water and soil samples.
For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
Bioassay Systems EnzyChrom™ Glucose Oxidase Assay Kit. For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, FL530/585nm. Samples: Cell/tissue lysate, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 (OD), 0.002U/L (FL).
Bioassay Systems EnzyChrom™ Ketone Body Assay Kit. For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism.
For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Uses 10 µL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day. Method: OD340nm. Samples: Serum, plasma, urine etc. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.12 mM.
Bioassay Systems QuantiChrom™ Acetylcholinesterase Assay Kit. For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.
For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors. Key Features: Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD412nm. Samples: Blood, serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 10 U/L.
Bioassay Systems QuantiChrom™ Iron Assay Kit. For quantitative determination of iron ions Fe3+ and/or Fe2+ and evaluation of drug effects on iron metabolism.
For quantitative determination of iron ions Fe3+ and/or Fe2+ and evaluation of drug effects on iron metabolism. Key Features: Sensitive and accurate. Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Biological (e.g. serum) and environmental samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 27 µg/dL (4.8 µM).
Bioassay Systems QuantiChrom™ Magnesium Assay Kit. For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.
For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).
Bioassay Systems EnzyChrom™ AF Cholesterol Assay Kit. For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 10 mg/dL cholesterol for colorimetric assays and 0.02 to 2 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.
Bioassay Systems EnzyChrom™ Aspartate Transaminase Assay Kit. For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.
For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity. Key Features: Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.
Bioassay Systems EnzyChrom™ Creatine Kinase Assay Kit. For quantitative determination of creatine kinase activity and evaluation of drug effects on CK activity.
For quantitative determination of creatine kinase (CK) activity and evaluation of drug effects on CK activity. Key Features: Sensitive and accurate. Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 min and 40 min at room temperature or 37°C. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 U/L.