Applied Biosystems
Applied Biosystems™ integrated systems for sequencing and real-time, digital and endpoint PCR have been trusted by the world's top scientists for over 20 years. From sample prep to data analysis, we provide expert solutions and dedicated support.
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Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls
VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Content And Storage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Form | Liquid |
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 5-plex |
| Detection Method | Primer-probe |
| Includes | dUTP |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Antibody-based |
| Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Product Line | TaqPath™ |
Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit
The MagMAX Pro HT NoSpin Plasmid Miniprep Kit enables scalable, ultra-rapid purification of high-quality, transfection-grade plasmid DNA (pDNA) from bacterial cultures. Using magnetic-bead technology, it simplifies workflows by eliminating centrifugation and vacuum filtration bottlenecks.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | 2°C to 8°C BactBind Beads, PlasmidPure Beads, Endotoxin Reduction Buffer 15°C to 25°C RNAse A, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, Wash 1 Buffer, Wash 2 Buffer, Elution Buffer |
|---|---|
| Isolation Technology | Magnetic Bead |
| Purity | Transfection Grade |
| Sample Volume | Bacterial Culture, 1 mL per well |
| Quantity | 1 Ea. |
| Green Features | Energy-efficient design, reduced packaging, and elimination of volatile organic solvents |
| Scale | Mini |
| For Use With (Application) | Cloning, Transfection, Protein Expression, Protein Production, mRNA production via In Vitro Transcription, Gene Editing, Sequencing, PCR, Nucleic acid labeling, PCR |
| Size Distribution | 5-12kb |
| Test Time | ~ 32 min. for 96 samples, 1 KingFisher 96 DWP |
| Plate Compatibility | KingFisher 96 Deep Well Plates with or without barcodes |
| Format | 96 Deep-well Plate |
| Product Type | Automated, pDNA extraction kit |
| For Use With (Equipment) | KingFisher Apex, Flex and Presto Processors with 96 Deep Well Heads, Liquid Handling Robotic Systems |
| Sample Type | Bacteria, E. coli |
| Yield | Up to 20 μg pDNA using high copy plasmid |
| Nucleic Acid Type | Plasmid DNA (pDNA) |
| Elution Volume | 100 μL |
Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA)
Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Purity or Quality Grade | LD (Low DNA) |
| Quantity | 250 units |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq DNA Polymerase |
| No. of Reactions | 200 Reactions |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Applied Biosystems™ MagMAX™ Pure Bind Beads
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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MagMAX Pure Bind Beads are ready-to-use magnetic beads that provide optimized size selection and PCR cleanup, removing small fragments such as dNTPs, salts, primers, and primer-dimers.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Form | Beads in Suspension |
| For Use With (Application) | NGS Cleanup, PCR Cleanup, PCR Purification, DNA Purification, Sequencing |
| Test Time | 20 min. |
Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 10 X |
| Reverse Transcriptase | MultiScribe™ |
| Final Product Type | cDNA |
| Concentration | 10X |
| For Use With (Application) | miRNA analysis |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Size (Final Product) | 200 bp or less |
| Reaction Format | Separate Components |
| Product Type | MicroRNA Reverse Transcription Kit |
| Sample Type | RNA |
| Reaction Speed | Fast |
| Optimal Reaction Temperature | 42°C |
| Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Genotyping Target | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
| Product Type | Genotyping Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 |
| Sample Type | DNA (Genomic) |
| Reaction Speed | Standard |
| Technique | SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation) |
| Product Line | TaqMan™ |
Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup
ExoSAP-IT™ Express PCR Product Clean-up Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. This enzymatic clean-up method offers precision, increased yield, and a faster turnaround time compared to alternative purification methods, such as spin columns or beads.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | Shipped on dry ice. Store at -20°C. |
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Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers
POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Accuracy | 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary) |
|---|---|
| Product Type | Polymer |
| For Use With (Application) | Standard Sequencing, Fragment Analysis (Denaturing) |
| Test Time | 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.) |
| Product Line | POP-7™ |
Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit
The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | 8-well Strip,96-well Plate |
| Product Type | Direct Cycle Sequencing Kit |
| Technique | Fluorescent Dye Terminator Sequencing |
| For Use With (Application) | Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes) |
| Template Compatibility | BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes) |
| Product Line | BigDye™ |
Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Format | 12-well Strips,8-well Strip,96- or 384-well Plate |
|---|---|
| Product Type | Sequencing Buffer |
| For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
| Technique | Fluorescent Dye Terminator Sequencing |
| Product Line | BigDye™ Terminator |
Applied Biosystems™ POP-6™ Polymer Dx
POP-6 Polymer Dx is pre-formulated in a single-use pouch.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Store the polymer pouch at 2-8°C until ready to use. Note that expired pouches cannot be used on the instrument. On-instrument life/usage 3500 Dx (8 capillary) Genetic Analyzer: lesser of 7 days or 384 samples or 60 injections 3500xL Dx (24 capillary) Genetic Analyzer: lesser of 7 days or 384 samples or 20 injections The pouch includes additional polymer volume to accommodate both installation and a reasonable number of post-installation wizard operations such as bubble removal without affecting the number of samples/injections available. |
| Capillary Length | 50 cm, 36 cm |
| Test Time | 2 hr. for 50 cm capillary (long read), 1 hr. for 36 cm capillary (std. seq.) |
| Product Line | POP-6™ |
Applied Biosystems™ POP-7™ Polymer, for 3500/SeqStudio™ Flex
POP-4, POP-6, and POP-7 are conveniently offered in easy to use pouch packages. The POP-7 separation matrix is optimized for short- to long-read sequencing and fragment analysis.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Application) | Long-Read Sequencing, Short Read Sequencing, Fragment Analysis (Denaturing) |
|---|---|
| Product Line | POP-7™ |
Applied Biosystems™ Shrimp Alkaline Phosphatase (SAP)
Shrimp Alkaline Phosphatase (SAP) is a high specific activity, heat-labile alkaline phosphatase purified from a recombinant source and originally isolated from Pandalus borealis (arctic shrimp).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Enzyme | Alkaline Phosphatase |
|---|---|
| Concentration | 1 unit/μL |
| For Use With (Application) | Dephosphorylation of DNA/RNA |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Product Type | Multiplex Kit |
|---|---|
| For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
| Sample Type | DNA |
| Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
| Product Line | SNaPshot™ |
| Detection Method | Primer-probe |