AdipoGen Bromocresol Green
Chemical. CAS 76-60-8. Formula C21H14Br4O5S. MW 698.01. Bromocresol green BCG is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualization of the compounds with functional groups whose pKa is below 5.0. One major use is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. In aqueous solution, bromocresol green will ionize to give the monoanionic form yellow, that further deprotonates at higher pH to give the dianionic form blue. Visual transition interval pH 3.8 yellow - 5.4 blue. UV-Visible lambdamax 423 nm, 444 nm, 617 nm.