Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt | 82207-62-3 | MFCD00043438 | 100g
HEPES potassium salt | Purity: 99.5% | Mol Wt: 273.69 | 82207-62-3 | MFCD00043438 | 100g
Medchemexpress LLC Bicine | 150-25-4 | 98.0% | 10 G
Bicine is a buffer utilized in physiological research. It is a derivative of the simple amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection. This product appears as a white to off-white solid.
- Buffer for physiological research
- Derivative of amino acid glycine
- Enhances sensitivity of guanosine enzyme histochemical detection
- White to off-white solid appearance
- Molecular weight: 163.17
- Chemical formula: C6H13NO4
- Shipped at room temperature
- Stable at room temperature for 3 years
- Stable in solvent at -80°C for 2 years or -20°C for 1 year
- For research use only
Research Products International Corp TES, Sodium Salt, 25 G
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Apexbio Technology LLC HEPES(Synonyms: HEPES buffer, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid), 250g, CAS: 7365-45-9.
HEPES (CAS 7365-45-9) is a zwitterionic sulfonic acid buffer commonly utilized in biomedical research due to its capacity to maintain physiological pH stability within a buffering range of approximately pH 6 8 to 8 2 Mechanistically HEPES modulates intracellular pH balance thereby promoting lysosomal biogenesis and supporting associated cellular processes It is routinely employed in cell culture systems to precisely regulate environmental conditions and is relevant in biochemical assays involving specific cellular signaling pathways and intracellular pH-dependent phenomena
Alkali Scientific MES Buffer, Anhydrous, 250 G
MES Buffer, Anhydrous, 250g, is a biochemical reagent used for pH buffering in laboratory applications, including enzyme assays, protein purification, and cell culture. The anhydrous form of MES ensures a dry, stable reagent free from moisture that could affect experimental outcomes. This 250g size is ideal for medium-scale applications, providing enough buffer for multiple experiments without the need for frequent replenishment. MES provides a consistent pH environment that is essential for biochemical reactions where pH fluctuations can interfere with results. It is commonly used in molecular biology, biochemistry, and biotechnology studies where precise pH control is crucial.
Bioworld ACES Buffer, 0.5M, pH 7.5, 250 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Gold Biotechnology Inc HEPES Sodium Salt 1 kg
HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups such as Tris This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers This buffer is capable of forming radicals and is therefore not suitable for redox reactions It is suitable for use in the bicinchoninic acid (BCA) assay
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 6.5, 250 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltpH: useful range: 6.0 - 7.2Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. PH Value: : 6.0 - 7.2
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660189.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 1 Kg
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent supplied in a 1 kg bottle. This high-quality buffering agent is commonly used in laboratory and industrial applications for maintaining stable pH conditions, particularly for enzyme assays, protein studies, and cell culture applications. HEPPS is particularly useful in biological research, as it maintains pH stability under a wide range of experimental conditions. Its use in protein purification and electrophoresis helps researchers obtain precise and consistent results. This 1kg bottle is ideal for high-throughput laboratories or research settings requiring large quantities of buffering solution.
Medchemexpress LLC BES sodium | 66992-27-6 | 97.0% | 235.23 | 500 G
BES sodium is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is used in the diagnostic testing area and appears as a white to off-white solid. This product is for research use only.
- Secondary standard biochemical buffer
- Effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C)
- Used in diagnostic testing
- White to off-white solid
- For research use only
Apexbio Technology LLC MES sodium salt 71119-23-8 500mg
MES sodium salt (CAS 71119-23-8) is a buffering agent designed to maintain stable pH conditions in the range of 5 5 7 0 supporting physiological experimental environments MES sodium salt does not have a direct cellular or molecular target and is not a small-molecule inhibitor agonist or antagonist Its key role is pH stabilization in various biological and biochemical assays including enzymatic activity assays receptor pathway investigations and isolation of cellular organelles or biomolecules Experimental models include plant cell culture systems and physiological in vitro assays Based on these properties MES sodium salt holds research potential in applications such as plant cell culture controlled metabolic pathway studies and signal transduction research requiring consistent pH conditions
Boston Bioproducts Inc Phosphate Buffered Saline-Tween 10X, 4 x 1 L bottles, 0.5% Tween-20, pH 7.4, liquid
Phosphate Buffered Saline-Tween 10X, 4 x 1 L bottles, 0.5% Tween-20, pH 7.4, liquid