Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
MilliporeSigma END COVER PINCH PIPES,3/8X 5/8
NovaSeal™ End cover pinch pipes, 3/8 in. ID x 5/8 in. OD
Hampton Research HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml
HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | 90217-02-0 | MFCD00467521 | 10MG
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | Purity: >=85% (HPLC) | Mol Wt: 311.77 | 90217-02-0 | MFCD00467521 | 10MG
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.4, Low Endotoxin) - 250ml
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical immunoassay as a wash buffer. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration and tested for endotoxin.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.0) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt >=99% (titration) | 103404-87-1 | 100G
HEPES hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 249.3 | 103404-87-1 | 100G
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt | 103404-87-1 | | 25g
HEPES hemisodium salt | Purity: 99% | MW: 249.3 | 103404-87-1 | 25g
Alkali Scientific CellPro™ Tris-HCl Buffer, 1M, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
