Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 10mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Apexbio Technology LLC Annexin V-HF488/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes 7-AAD can specifically bind to double-stranded DNA resulting in red fluorescence Normally 7-AAD cannot pass through intact cell membranes however it can penetrate the cell membranes of late-stage apoptotic and dead cells staining their nuclei Combined staining with Annexin V-HF488 and 7-AAD allows differentiation of cells in various stages of apoptosis and dead cells
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (HF594) 100tests
EdU Flow Cytometry Assay Kits (HF594) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The EdU Flow Cytometry Assay Kits (HF594) use HyperFluor 594 azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (HyperFluor 594 azide excitation maximum at 590 nm emission maximum at 617 nm)
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.
Growcells PBS 10X 10L EA
Sterile 10X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 80g, KCl 2g, Na2HPO4 14.4g, KH2PO4 2.4g
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Boston Bioproducts Inc 10X Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - 10X Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases capable of degrading proteins in the extracts The most effective strategy to enhance the yield of intact proteins is through the addition of inhibitors targeting these known endogenous enzymes The Protease Inhibitor Cocktail is intended for the purification of His-tagged proteins to improve protein stability This cocktail functions by inhibiting proteases that degrade either phosphorylated or non-phosphorylated protein substrates This Protease Inhibitor Cocktail contains individual components including AEBSF Bestatin E-64 Phosphoramidon and Pepstatin A offering broad-spectrum specific inhibition against cysteine serine aspartic thermolysin-like proteases and aminopeptidases It has been optimized and validated specifically for use with His-tagged proteins The cocktail is dissolved in DMSO and supplied as a ready-to-use solution
S2 Media 100mL 1x Phosphate Buffered Saline (PBS), pH 7.5, 125mL PP Boston Round Bottle, 1ea
1x Phosphate Buffered Saline (PBS), pH 7.5 has many applications due to its isotonic nature, including uses as a rinse fluid, diluent, and buffer. Phosphate salts are nontoxic to living cells and have a high buffering capacity. pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. PBS is generally used as a 10mM working solution containing a mixture of sodium chloride and inorganic phosphate salts.• Product meets AOAC, APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• PBS should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-024100 100ML
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in DPBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.50% Trypsin. EDTA: 0.20% (5.3 mM) EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN P10-021100 100ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Fd Neurotechnologies Inc PHOSPHATE-BUFFERED 4% PARAFORM
This 1000ml filtered and ready-to-use tissue fixative is freshly made from high purity paraformaldehyde powder. This solution may be stored at room temperature for at least one year without change of the original quality. The standard solution contains 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). Other concentrations can also be provided upon request.