Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid | MFCD00007532 | 250G
N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid, MFCD00007532, 250G
Medchemexpress LLC 2-methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.4% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptor agonist, with EC50s of 19, 6.2, and 5 nM for human P2Y13, mouse P2Y13 and human P2Y12, respectively. It has pEC50s of 8.29 and 5.75 for human P2Y1 and rat P2Y6, respectively. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptor agonist
- Acts on human P2Y13, mouse P2Y13, human P2Y12, human P2Y1 and rat P2Y6 receptors
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1
Alkali Scientific MES Buffer, Anhydrous, 100 G
MES Buffer, Anhydrous, 100g, is a highly effective biochemical reagent used for pH buffering in laboratory research. The anhydrous form of MES ensures that the buffer is stable and free from moisture, making it ideal for sensitive reactions where precise control of pH is necessary. MES is widely used in enzyme assays, protein purification, and cell culture applications, where it maintains a stable pH environment. The 100g volume is suitable for small-scale experiments, providing enough reagent for short-term studies or pilot projects. MES Buffer, Anhydrous, is an essential reagent for maintaining consistent pH in a variety of biochemical and biological applications.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration25KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659775.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Sodium Salt is a biochemical reagent available in a 500g size, ideal for large-scale applications in biological and chemical research. It is widely used to maintain pH stability in biological systems, particularly in cell culture, protein assays, and enzyme reactions. HEPES sodium salt provides reliable buffering capacity in the pH range of 6.8–8.2, ensuring that experimental conditions remain consistent. It is non-toxic to cells, supporting reproducible results in long-term experiments. This bulk package is perfect for high-throughput laboratories, where large quantities of buffer are required for routine cell culture and biochemical analysis procedures.
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659982.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 250 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent and buffering agent used to prepare stable pH environments in laboratory experiments. The 250g quantity is suitable for large-scale applications and long-term use in various research projects. BES is often employed in biochemical assays, including enzyme activity studies, protein analyses, and molecular biology experiments, where maintaining a stable pH is critical. It is highly valued in protocols requiring accurate and consistent pH control, such as electrophoresis and buffer preparation for biological applications. BES is indispensable for labs focused on research that demands reliable buffering for precise biochemical reactions.
eMolecules 7365-45-9 | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | Chem-Impex | 7365-45-9 | MFCD00006158 | 238.300 | C8H18N2O4S | 100.000 | OCCN1CCN(CCS(O)(=O)=O)CC1 | 250g | 603112820
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659941.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 500 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 500g volume is a biochemical reagent used for pH buffering in various laboratory applications, particularly where enhanced ionic strength is required. This sodium salt form is ideal for experiments that demand robust pH control, such as in cell culture, enzyme assays, and protein purification. The 500g size is ideal for medium-scale laboratory use, providing enough reagent for multiple experiments or ongoing studies. MES Sodium Salt is non-interfering, making it suitable for sensitive biological systems and molecular biology studies where maintaining a stable pH is crucial for accurate results.
Medchemexpress LLC HEPES (sodium) | 75277-39-3 | MFCD00036463 | 99.9% | 260.29 | 250 MG
HEPES sodium is a nonvolatile zwitterionic chemical buffering agent broadly applied in cell culture. It is effective at pH 6.8 to 8.2 and is also a potent inducer of lysosome biogenesis.
- Nonvolatile zwitterionic chemical buffering agent
- Broadly applied in cell culture
- Effective at pH 6.8 to 8.2
- Potent inducer of lysosome biogenesis
- Maintains superhydrophilicity of titanium for at least 3 months, resulting in continuous retention of bioactivity and osteoconductivity
- Drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, and disrupts global cellular transcriptional profiles, leading to the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells
Sigma Aldrich Fine Chemicals Biosciences DIPSO BioXtra pH 4.0 5.5 2100G
DIPSO BioXtra pH 4.0 5.5 2100G
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660290.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 25 Kg
HEPES, Free Acid is a widely used biochemical reagent, available in a 25kg package for larger-scale applications. This highly stable buffer is effective in maintaining a pH range of 6.8–8.2, making it ideal for a variety of biochemical and biological applications such as protein analysis, cell culture, and drug formulation. HEPES helps maintain physiological pH levels, preventing changes that could interfere with experiments. This bulk package is especially beneficial for laboratories requiring frequent buffer preparation, allowing for efficient cost-saving and consistent results across a wide range of biological and chemical protocols.
Biotang Inc HEPES-Buffer, 0.5M pH 7.6, 100ml 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 7.6 +/- 0.15. BB-2078
HEPES-Buffer, 0.5M pH 7.6, 100ml 500 mM HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), pH 7.6 +/- 0.15. BB-2078.
eMolecules 29915-38-6 | TAPS | Ambeed | MFCD00007538 | 243.270 | C7H17NO6S | 98.000 | OCC(CO)(CO)NCCCS(O)(=O)=O | 5g | 600837773
TAPS | Ambeed | 29915-38-6 | MFCD00007538 | 243.270 | C7H17NO6S | 98.000 | OCC(CO)(CO)NCCCS(O)(=O)=O | 5g | 600837773