Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005335 HEPES EMPROVE EVOLVE 100G
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 100 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 250G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 250G
eMolecules 913642-78-1 | tert-Butyl 2-(2-hydroxyethyl)morpholine-4-carboxylate | ChemScene | MFCD09608014 | 231.292 | C11H21NO4 | 97.000 | CC(C)(C)OC(=O)N1CCOC(CCO)C1 | 100mg | 596346186
tert-Butyl 2-(2-hydroxyethyl)morpholine-4-carboxylate | ChemScene | 913642-78-1 | MFCD09608014 | 231.292 | C11H21NO4 | 97.000 | CC(C)(C)OC(=O)N1CCOC(CCO)C1 | 100mg | 596346186
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 5 MG
AR-13324 analog mesylate is an analog of AR-13324. This product is for research use only.
- Acts as a Rho-associated protein kinase (ROCK) inhibitor.
- Functions as a norepinephrine transporter (NET) inhibitor.
Medchemexpress LLC MOPS sodium salt | 71119-22-7 | 99.8% | 500 G
MOPS sodium salt is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It significantly improves the thermal stability of BSA. MOPS sodium salt is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
- Purity: 99.8%
- Appearance: Solid
- Color: White to off-white
- Storage: 4°C, sealed, away from moisture. In solvent: -80°C for 6 months; -20°C for 1 month (sealed, away from moisture).
- Solubility in H2O: 100 mg/mL (needs ultrasonic)
- Solubility in DMSO: 50 mg/mL (needs ultrasonic; use newly opened DMSO)
Medchemexpress LLC 2-Morpholinoethanesulphonic acid potassium | 39946-25-3 | 99.9% | 50 G
MES potassium is an amphoteric ion buffer widely used in biochemistry and molecular biology experiments. It provides effective buffering capacity around pH 6.15 and is suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Features a buffer capacity ranging from pH 5.5-7.0
- Functions as a zwitterionic buffer
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions, suitable for metal ion studies
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659860.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 25 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent used as a buffering agent in laboratory applications. The 25g quantity is ideal for smaller experiments or laboratories with limited storage space. BES is known for its effective pH buffering capabilities, making it an essential component in protocols that require precise pH control, such as protein purification, electrophoresis, and enzyme assays. It helps maintain the stability of biochemical reactions and is frequently used in molecular biology, biochemistry, and medical research where consistent pH levels are critical for experimental success. The small size is ideal for smaller-scale experiments or specialized applications.