Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (20X) - 500ml
Phosphate Buffered Saline (PBS) is a buffer solution widely used in biological research due to its isotonic and non-toxic nature. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for applications including protein purification, Western Blotting and immunoassays, sample dilution, and as a transport medium. This buffer is formulated in a 20X concentration, and can be diluted to support its application.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
eMolecules tert-butyl 3-(hydroxymethyl)-3-methylazetidine-1-carboxylate | 1363382-91-5 | MFCD20926177 | 1g
Pharmablock | tert-butyl 3-(hydroxymethyl)-3-methylazetidine-1-carboxylate | 1g | 551132908 | PBN20120070 | | 1363382-91-5 | MFCD20926177 | 201.266 | C10H19NO3
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eMolecules tert-Butyl 4-(hydroxymethyl)benzoate | 143726-85-6 | MFCD04973449 | 1g
Ambeed | tert-Butyl 4-(hydroxymethyl)benzoate | 1g | 592282779 | A321260 | | 143726-85-6 | MFCD04973449 | 208.257 | C12H16O3
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Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 1 KG
MOPS sodium salt is a buffering agent used in biology. It maintains the pH of mammalian cell culture media and significantly improves the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH in mammalian cell culture media.
- Improves thermal stability of BSA.
Medchemexpress LLC 1-Propanesulfonic acid, 3-(bis(2-hydroxyethyl)amino)-2-hydroxy- | 68399-80-4 | MFCD00038353 | 98.0% | 100 G
DIPSO is a biological zwitterionic buffer with a useful pH range of 7.0 to 8.2. It can interfere with meiotic regulation in mouse oocytes and exhibits surfactant activity at 10 mM. This product is for research use only.
- Useful pH range of 7.0 to 8.2
- Can interfere with meiotic regulation in mouse oocytes
- Exhibits surfactant activity at 10 mM
![eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 220598-43-6 | MFCD18073347 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159302.JPG-250.jpg)
eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 220598-43-6 | MFCD18073347 | 1g
Pharmablock | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 1g | 551175136 | PBZ0195 | | 220598-43-6 | MFCD18073347 | 213.277 | C11H19NO3
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Sigma Aldrich Fine Chemicals Biosciences Piperazine BioUltra, anhydrous, >=99.0% (T) | 110-85-0 | MFCD00005953 | 25G
Piperazine BioUltra, anhydrous, >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 25G
Sigma Aldrich Fine Chemicals Biosciences Piperazine | 110-85-0 | MFCD00005953 | 100 g
Piperazine | Purity: ≥99.0% | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 100 g