Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp MES, Anhydrous, 1 Kilograms
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Bioworld EGTA 0.5M, PH 8.0,250ML
EGTA 0.5M, pH 8.0, DNase and RNase Free,250ml.EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
S2 Media 100mL 1x Phosphate Buffered Saline (PBS), pH 7.5, 125mL PP Boston Round Bottle, 1ea
1x Phosphate Buffered Saline (PBS), pH 7.5 has many applications due to its isotonic nature, including uses as a rinse fluid, diluent, and buffer. Phosphate salts are nontoxic to living cells and have a high buffering capacity. pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. PBS is generally used as a 10mM working solution containing a mixture of sodium chloride and inorganic phosphate salts.• Product meets AOAC, APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• PBS should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.
Boca Scientific Inc BuccalFix DNA/RNA Stabilization Buffer, 25ml, Sufficient for Stabilizing 50 swabs
BuccalFix Stabilization Buffer is designed to completely stabilize the DNA and RNA on buccal swabs by inhibiting all enzymatic and microbial activity that occurs following buccal sampling. DNA can be stored in BuccalFix at ambient temperature for over 2 years, while RNA can be stabilized for 4 days. While stabilized, the structure and integrity of the DNA and RNA is fully maintained for further downstream processing applications. Stabilized DNA in BuccalFix can be processed for isolation by the addition of Proteinase K and subsequent isolation steps.
Crescent Chemical Co Inc TRIS(HYDROXYMETHYL)AMINOMETHAN
Tris(hydroxymethyl)aminomethane.hydrochloride molecular biology grade DNase/RNase Protease not detected. Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 39787.01 CAS 1185-53-1
ILEX LIFE SCIENCES LLC Promed Bioscience Atelocollagen Ultrapure, Porcine Type I, Enzymatic extraction, dialyzed, lyophilized, 10 mg
Atelocollagen Ultrapure, Porcine Type I, Enzymatic Extraction, Dialyzed, Lyophilized. Characterized by rich and ultra dialysis-purified Type I collagen content in a regular fibril-like configuration that retains its superior functionality and structural properties. The proprietary pepsin enzyme-based extraction removes telopeptides at the nonhelical N and C termini, resulting in a triple helix configuration that exhibits reduced immunogenicity. APPLICATION: Biocompatible and biodegradable, suitable for many research applications, such as bioink for 3D bioprinting, in vitro cell culture, cell functional assays, etc. MANUFACTURER: Promed Bioscience Ltd. CAS NO.: 9007-34-5. PURITY: greater than 97%. SOURCE: Porcine tendon. AMINO ACID ANALYSIS: Typical profile. STERILITY TESTING: Pass (0 cfu/g). STERILIZATION METHOD: Produced under strict aseptic conditions. CELL ATTACHMENT ASSAY: Pass. CELL TOXICITY ASSAY: Pass. OTHER NAMES: Type 1 Collagen, Collagen-1.
Enzo Life Sciences Physiological saline (NaCl 0.9%) (endotoxin-free) (sterile) (1.5ml)
Sterile and endotoxin-free physiological saline for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
Gold Biotechnology Inc HEPES, Free Acid 250 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
Pickering Laboratories Inc CHEM LITHIUM ELUENT 4X1QT/CS
Lithium Eluant, pH 7.50, case of 4 (950 mL/bottle) Amino Acid Analysis.
Sigma Aldrich Fine Chemicals Biosciences PIPES sodium salt >=99% (titration) | 10010-67-0 | MFCD00065472 | 100G
PIPES sodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 324.35 | 10010-67-0 | MFCD00065472 | 100G