Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biocare Medical, LLC PBS Plus, 10X
For laboratory use and procedures requiring a high quality phosphate buffered saline with superior pH stability. PBS Plus (sodium azide free) buffer works well for manual and automated histopathology and immunohistochemistry (IHC) applications with the addition of a surfactant.
Enterprise Technology Solutions Expanding File Jan-Dec, 9" Expansion, 12 Sections, 1/12-Cut Tab, Letter Size, Brown, Elastic Cord
ABILITYONE 7530016819290 Skilcraft Increase your efficiency with this letter-size expanding file featuring 12 tabs for monthly filing. The accordion-style pocket folder is covered with soil-resitant leather-patterned paper and is durable to stand up to heavy handling.
Apexbio Technology LLC Annexin V-APC/7-AAD Apoptosis Kit 20tests
Annexin V-APC/7-AAD Apoptosis Kit is possible to differentiate cells at various stages of apoptosis and distinguish dead cells Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit contains the nuclear dye 7-AAD a fluorescent probe that specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot permeate intact cell membranes However cells in the late apoptotic or necrotic stages exhibit compromised membrane integrity allowing 7-AAD to enter and stain the nuclei
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS - 1L
Dulbecco s Phosphate Buffered Saline DPBS - 1L (For Research Use and Further Manufacturing Only)
Enzo Life Sciences PBS (endotoxin-free) (sterile) (10ml)
Sterile and endotoxin-free PBS for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
Biotang Inc Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Ethos Biosciences Dilurex PBS Casein Sucrose Blocker, 100ml, Ethos
Dilurex PBS Casein Sucrose Blocking Buffer offers the best price to performance ratio on the market. The base system is phosphate buffered saline - perfect for systems that are using PBS as a diluent or reaction buffer. Casein is used as the blocking protein which binds to unoccupied hydrophobic or charged sites on assay surfaces preventing unwanted non-specific binding of antibodies, enzymes, or other proteins leading to clearer, more reliable signals. Sucrose stabilizes your mixture by preserving the folding integrity of proteins during drying or freezing. Choose one of our other blocking buffers if your assay is detecting phosphoproteins, uses biotin/streptavidin detection, Alkaline Phosphatase detection, autoimmune assays, or aldehyde coated substrates in case of potential interference.
Medchemexpress LLC Pbs/cds Qds | 5 MG
PbS/CdS QDs (PbS/CdSe QD) is a fluorescent probe that can be used for in vivo fluorescence imaging in the second near-infrared window. The 2nd-NIR fluorescence imaging at ca.1300 nm using the PbS/CdS QDs results in the highest signal to background ratio with a low blurring for in vivo imaging.
- Fluorescent probe for in vivo fluorescence imaging in the second near-infrared window
- Highest signal to background ratio with low blurring for in vivo imaging at ca.1300 nm
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Bioworld Carbonate-buffered saline pH 9.5 (5X), 500 mL
This buffer contains 125mM sodium carbonate and 750mM sodium chloride, pH 9.5. The buffer is important for several different cellular processes. It is one of the major buffering systems used to maintain the pH of mammalian blood and is also important in the acid-base homeostasis of living things, including humans. It tends to maintain a relatively constant plasma pH and counteract any force that would alter it.Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption. Appearance: LiquidPH Value: 9.5
AG Scientific Inc Hygromycin B in PBS, 10X2 ML
The concentration of Hygromycin B in PBS buffer is 50 mg/ml. Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.CAS Number: 31282-04-09Molecular Weight: 527.53Chemical Formula: C20H37N3O13Solubility: Soluble in WaterStorage Temperature: 2-8CResearch or further manufacturing use only, not for food or drug use.
Promocell GmbH PromoCell Dulbeccos PBS 500 ml, w/o Ca++/ Mg++
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.Dulbecco's PBS can be purchased with or without Ca^2 ^/ Mg^2 ^.
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Apexbio Technology LLC Annexin V-PE Apoptosis Kit 20tests
Annexin V-PE Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Alternatively you can directly use our ready-to-use dual-staining kits Annexin V-PE/7-AAD Apoptosis Kit ( K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)