Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10400-1000.0.JPG-250.jpg)
Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
PBS 1X Solution (Phosphate-Buffered Saline) is a balanced, isotonic buffer commonly used in biological and biomedical research. Prepared at a working concentration, it contains sodium chloride, sodium phosphate, and potassium phosphate to maintain pH and osmolarity. It is widely used in cell culture, molecular biology, and immunohistochemistry for cell washing, reagent preparation, and sample dilution. PBS helps preserve the integrity and viability of biological samples by providing a stable, non-disruptive environment. Its compatibility with most biological systems makes it a fundamental laboratory reagent.
Pickering Laboratories Inc LITHIUM ELUANT 2.75 4X950ML
Lithium Eluant, pH 2.75, case of 4 (950 mL/bottle) Amino Acid Analysis.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Rockland Immunochemicals 1.0 M Tris HCl pH 8.5 - MB-027-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Biotang Inc Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 1 Kilogram
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Boston Bioproducts Inc PIPES Buffer (1 M, pH 6.8) - 500ml
PIPES Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Glycine-Native Running Buffer (10X) - 1L
Tris-Glycine-Native Running Buffer (10X) - 1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)