Used in Modified Alizarin Red S for Fetal Specimens for Minute bones and fetal ossification in mammalian embryos. References: Cumley, R.W., Crow, J.F., and Griffin, A.B., Stain Techn., 14:7 – 11, 1939, Dawson, A.B., Stain Techn., 1:123 – 124, 1926, Richmond, G.W. and Bennett, L., Stain Techn., 13:77 – 79, 1938, and Clark, G., (ed), Staining Procedures, 3rd ed., William & Wilkins, Baltimore, p. 138, c. 1973