TMPD dihydrochloride is a readily oxidizable compound and an enzymatically convertible redox-active substrate molecule. It also functions as an electron donor, serving as a reducing cosubstrate for heme peroxidases, and is a complex IV substrate.
- Utilized in cell culture and microbiology to differentiate organisms with cytochrome c oxidase activity.
- Distinguishes between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria.
- Oxidation by microorganisms produces a radical cation with a characteristic deep blue color.
- Successful colorimetric indicator for bacterial oxidases.
- Electrochemical recognition of oxidation can be applied to various pathogens.