Biotin and derivatives
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Apexbio Technology LLC Phos binding reagent (Phosbind) Biotin LC 50mg
Phosbind Biotin LC is a biotin-labeled dinuclear Zn(II) complex that selectively binds phosphorylated molecules at neutral pH (Kd 25 nM to phenyl phosphate) It is widely used in phosphoproteomics workflows to detect phosphoproteins on PVDF membranes via chemiluminescence (with streptavidin-HRP) or enrich phosphorylated peptides/proteins using streptavidin-agarose columns Compared to standard prestained protein markers (e g F4005 with 11 bands from 10 180 kDa) Phosbind enables phospho-specific detection Compatible buffers 20 mM Tris 150 mM NaCl pH 7 5
