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Cycloheximide is a protein synthesis inhibitor in eukaryotic cells. It prevents ribosomal mRNA translocation during protein translation. Cycloheximide has a variety of applications. It can be used to study cellular processes and to determine the half-life of a protein using western blotting techniques of protein cell lysate. It has been used to select for CHX-resistant strains of yeast and fungi. It also displays neuroprotective properties. Additionally, Cycloheximide has been used to inhibit yeast growth at concentrations of 200μM. It has been used to promote production of ethylene in leaves and fruit.
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