Incubator Accessories
Diagnostic Biosystems TISSUE PRIMER Tissue Primer
Tissue Primer is a universal enzyme-blocking reagent to use in horse radish peroxidase (HRP) and alkaline phosphatase (AP) based immunohistochemical staining procedures done on paraffin tissue sections, frozen tissue sections and cell smears to suppress nonspecific staining due to endogenous enzyme activity.\n\nIn the evaluation of tissue specimens by IHC using HRP and AP methods, the presence of endogenous enzymes can often obscure the specific staining of the target antigen. Endogenous peroxidase and AP activity can be inhibited by the use of Tissue Primer which will suppress endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in paraffin tissue sections, frozen tissue sections and cell smears.
Bellco Glass, Inc. Autofil 2 Bottle Top Filtration Device Full Assembly, 500 mL, 0.22 µm PES Unit, Sterile
Autofil 2 features the NEW High Flow Velocity 0.1µm, 0.2µm, and 0.45µm PES membranes. Perfect for filtering FBS and other viscous solutions. Our membrane is low protein binding and surfactant-free. Autofil 2 is available in 250mL, 500mL, and 1000mL complete units with the NEW EZlabpure media bottle or funnel only bottle-top filters. All units are sterile and manufactured from USP Class VI polystyrene in an ISO 13485 clean room, FDA regulated facility.Autofil 2 is compatible with the patented Autofil Pedestal and NEW Cradle Ring 2. These optional docking stations allow for hands free filtering and provide a stationary vacuum hose connection, allowing for efficient set-up and quick use while preventing spills and tissue culture hood contamination.
Diagnostic Biosystems PTEN PTEN (Tumor Suppressor)
PTEN gene is a tumor suppressor gene that maps to chromosome 10q23. PTEN, a novel tumor suppressor, functions as a regulator of both cell cycle progression and apoptosis ). Potentially, mutation and deletion of PTEN gene may result in a new signal transduction pathway related to human malignant tumors. Studies have demonstrated a reduction of PTEN expression in advanced breast cancers
Diagnostic Biosystems NKX31 NKX3.1
The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumorsuppressorthatis expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.
Diagnostic Biosystems P16 p16
P16 is a mitotic inhibitor protein. It competes with D-type cyclins to bind to cdk4 and cdk6. It acts as tumor suppressor and inhibits the progression of cells through the G1 phase of the cell cycle.
Diagnostic Biosystems S-100 S100 (4C4.9)
S100 belongs to the family of calcium binding proteins and S100A and S100B are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. This antibody stains with S-100 protein. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast,salivary and sweat glands, as well as glial cells. A large number of welldifferentiated tumors of salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. S100 is of great value in the identification of several neoplasms, particularly melanomas and their metastases.
Diagnostic Biosystems CD138 CD138 (B-A38)
CD138, also known as Syndecan-1, is a member of the transmembrane heparan sulfate proteoglycan family, acts as an extracellular matrix receptor and is involved in many cellular functions, including cell-cell adhesion and cell-matrix adhesion. CD138 expression is found in both hematopoietic and non-hematopoietic cells. In the hematopoietic system, CD138 labels plasma cells. It is an excellent marker for plasmacytic differentiation within the spectrum of hematologic malignancy. Among non-hematolymphoid cells, CD138 reactivity is observed in many types of epithelial cells and stoma cells in both normal and tumor tissues.
Diagnostic Biosystems NUCLEAR FAST RED FOR IHC Nuclear Fast Red
Nuclear Fast Red is a histological staining reagent suitable for visualization of nuclei in tissue sections. Nuclear Fast Red provides a red counterstain designed for optimal performance when used in immunohistochemistry.\n\nDBS’s Nclear Fast Red is intended for use in the histologic demonstration of nuclear staining. This staining technique is used to make the critical distinction between a normal nucleus and an abnormal one. When used as a counterstain in immunohstochemical procedures, Nuclear Fast Red is compatible with most commonly used chromogens including DBS HRP-Yellow, HRP-Green, HRP-Black, and DAB. Nuclear Fast Red is particularly useful with blue chromogens, such as DBS HRP-Blue.
Diagnostic Biosystems CD7 CD7 (CBC.37.80)
CD7 is expressed by most peripheral blood T cells, NK cells, and all thymocytes. It is one of the earliest surface antigens on T and NK-cell lineages. The antibody is a useful aid for classification of T-cell malignancies.
Diagnostic Biosystems PRAME PRAME
PRAME (Preferentially expressed Antigen in Melanoma) is a tumorassociated antigen and is a member of the family of cancer testis antigens (CTA). PRAME is expressed in malignant cells, including leukaemias, Hodgkin's lymphoma, breast cancer, and primary and metastatic melanomas. PRAME has low or no expression in normal tissues except for in testis, ovary, placenta, adrenals and endometrium.
Diagnostic Biosystems CYTOKERATIN 18 Cytokeratin 18 (DC10)
This antibody is specific to human cytokeratin 18 of 45 kDa. Cytokeratin 18 belongs to a family of acidic type A keratins and exists along with cytokeratin 8 in most simple ductal and glandular epithelia. This antibody does not react with squamous epithelium. It reacts with benign and malignant epithelial lesions as well as a majority of adenocarcinomas and basal cell carcinomas.
Diagnostic Biosystems PERMABLACK/HRP PermaBlack/HRP
PermaBlack/HRP is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing horseradish peroxidase. PermaBlack/HRP can be permanently mounted to produce a sharply contrasting ebony black color that can be easily distinguished from other stains.