Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences PyrexR VISTATM graduated c
Single metric scale with blue graduations.
Sigma Aldrich Fine Chemicals Biosciences PYREXR Disposable Glass Co
These disposable 5mL Pyrex glass centrifuge tubes are manufactured from Type I borosilicate glass and have a GPI 13-415 thread. They are supplied without caps. The tubes are designed to withstand centrifugation up to 3 000 RCf and are packed in convenient easy to use packs which are shrink-sealed for protection during shipment. for reusable phenolic screw caps with white rubber liners see CLS9999132 for reusable phenolic screw caps with Polytetrafluorethylene (PTfE) faced rubber liners see CLS999813 for disposable phenolic screw cap with rubber liner see CLS9999913.
Sigma Aldrich Fine Chemicals Biosciences TempliPhiTM 100 Amplificat
illustra(TM) TempliPhi(TM) DNA Amplification Kits use isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates.TempliPhi(TM) Kits use a unique process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi(TM) uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 C enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi(TM) protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.Two different types of TempliPhi(TM) DNA Amplification Kits are available to meet different throughput requirements. illustra(TM) TempliPhi(TM) DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- an
Sigma Aldrich Fine Chemicals Biosciences MicrospinTM G 50 Columns C
MicroSpin(TM) G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications including desalting buffer exchange removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.
Sigma Aldrich Fine Chemicals Biosciences PercollR Cytiva 17 0891 01
Percoll(R) is a well-referenced media for density gradient centrifugation of cells viruses and subcellular particles. Percoll(R) is composed of colloidal silica coated with polyvinylpyrrolidone (PVP).Percoll(R) is a low viscosity density gradient medium for preparation of cells subcellular particles and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 x g). The medium is available in easy-to-open resealable 250 mL and 1 l bottles.Percoll(R) consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). free PVP is present at only 1% to 2%. Percoll(R) is non-toxic almost chemically inert and does not adhere to membranes. Percoll(R) gradients can be formed within the density range of 1.0 to 1.3 g/mL and are iso-osmotic throughout.Percoll(R) can be stored unopened at room temperature for five years. At -20 C it can only be store
Sigma Aldrich Fine Chemicals Biosciences DuranR laboratory bottles
Reusable bottles and caps for the storage of reagents chemicals SOLUTIONons tissue culture media etc. Made of Duran borosilicate glass Type 3.3 for maximum chemical and thermal shock resistance.
Sigma Aldrich Fine Chemicals Biosciences HisTrapTM High Performance
HisTrap(TM) HP columns are prepacked with Ni Sepharose(R) HighPerformance and designed for simple high-reSOLUTIONon purificationof histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).HisTrap(TM) HP 1 mL and 5 mL columns are designed for simple one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose(R) High Performance which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.HisTrap(TM) HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification since (histidine)10 binds more strongly a higher concentration of imidazole can be added to the lysed ce
Sigma Aldrich Fine Chemicals Biosciences HisTrapTM High Performance
HisTrap(TM) HP columns are prepackedwith NiSepharose(R) High Performance and designed for simple high-reSOLUTIONon purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).HisTrap(TM) HP 1 mL and 5 mL columns are designed for simple one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose(R) High Performance which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.HisTrap(TM) HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification since (histidine)10 binds more strongly a higher concentration of imidazole can be added to the lysed ce
Sigma Aldrich Fine Chemicals Biosciences PyrexR solution bottle cap1EA
These Pyrex bottles are designed for storage of SOLUTIONons and are ideal for media preparation. Necks are tooled to increase mechanical strength and for uniform stopper fit.
Sigma Aldrich Fine Chemicals Biosciences Protein G High Performance
protein G HP SpinTrap(TM) is a prepacked single-use spin column containing protein G Sepharose(R) High Performance.
Sigma Aldrich Fine Chemicals Biosciences NAPTM 5 50 ST Cytiva 17 08
Disposable columns prepacked with Sephadex(R) G-25 DNA Grade and require only gravity to run.NAP(TM) Columns are disposable columns prepacked with Sephadex(R) G-25 DNA Grade and require only gravity to run. Each package includes a complete step-by-step protocol describing the fixed volumes needed for equilibration washing and sample elution.