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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 40 results
1

MilliporeSigma™ Supelco™ Ascentis™ Express Biphenyl Column, 2.7 μm Particle Size

The Ascentis™ Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Biphenyl
Phase Reversed Phase
Matrix Biphenyl Phase/Particle Platform (Fused-Core)
Endcapped Yes
Material Stainless Steel Hardware
Technique Fused-Core HPLC
Product Line Ascentis
2

MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 8
Technique Fused-Core HPLC
Product Line Ascentis
3

MilliporeSigma™ Supelco™ Ascentis™ Express OH5 HPLC Column, 5 μm Particle Size

Ascentis™ Express OH5 columns can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Hydroxy, Penta-
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
4

MilliporeSigma™ Supelco™ Ascentis™ Express C18 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm C18 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core™ particle design.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
5

MilliporeSigma™ Supelco™ Ascentis™ Express HILIC UHPLC Column, 2 μm Particle Size

Contains a bare silica stationary phase that can be used for traditional normal phase separations or aqueous normal phase chromatography.

Pore Size 90 Å
Particle Size 2 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Fused-Core Particle Platform
Endcapped No
Technique Fused-Core UHPLC
Product Line Ascentis
6

MilliporeSigma™ Supelco™ Ascentis™ Express C8 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
7

MilliporeSigma™ Supelco™ Ascentis™ Express C18 UHPLC Column, 2 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Particle Size 2 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core UHPLC
Product Line Ascentis
8

MilliporeSigma™ Supelco™ Ascentis™ Express C8 UHPLC Column, 2 μm Particle Size

Densely bonded, extensively endcapped dimethyloctyl stationary phase of Ascentis™ Express 2 μm C8 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Pore Size 90 Å
Particle Size 2 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core UHPLC
Product Line Ascentis
9

MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 5 μm Particle Size

The bare silica stationary phase of Ascentis™ Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
10

MilliporeSigma™ Supelco™ Ascentis™ Express C8 HPLC Column, 5 μm Particle Size

The densely bonded, extensively endcapped dimethyloctyl stationary phase of Ascentis™ Express 5 μm C8 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
11

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase
Matrix PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 1 to 9
Technique Fused-Core HPLC
Product Line Ascentis
12

MilliporeSigma™ Supelco™ Ascentis™ Express OH5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express OH5 columns can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Hydroxy, Penta-
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
13

MilliporeSigma™ Supelco™ Ascentis™ Express C18 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Stationary Phase C18 (octadecyl)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Material Stainless Steel
Max. Pressure 600 bar(9000 psi)
Column Format Analytical Column
Particle Size 2.7 μm
Type HPLC/UHPLC Column
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Packing Material Fused-Core particle platform superficially porous particle
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
USP Type USP L1
14

MilliporeSigma™ Supelco™ Ascentis™ Express OH5 UHPLC Column, 2 μm Particle Size

The Ascentis™ Express 2 μm OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry.

Pore Size 90 Å
Particle Size 2 μm
Type LC-MS Column
Stationary Phase Hydroxy, Penta-
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase/Fused-Core Particle Platform
Endcapped Yes
Technique Fused-Core UHPLC
Product Line Ascentis
15

MilliporeSigma™ Supelco™ Ascentis™ Express F5 UHPLC Column, 2 μm Particle Size

Ascentis™ Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Pore Size 90 Å
Particle Size 2 μm
Type LC-MS Column
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase
Matrix PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core UHPLC
Product Line Ascentis