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Cellular Engineering Technologies iPS Episomal Reprogramming Kit (1 vial)
Proprietary episomal plasmid mix where individual reprogramming factors (RFs) are placed in EBNA-1 containing vectors containing a CMV promoter with appropriate poly A sequences. This kit allows for high expression levels of RFs such as Oct-4, Sox-2, and Klf-4 while avoiding the oncogenic potential of transcription factors such as c-Myc, l-Myc, and Lin28. Along with these Yamanaka factors, the kit contains a p53 anti-sense element, which is necessary for preventing apoptosis in cells that are in the process of reprogramming, along with a Red Fluorescent Protein Reporter tag to determine transfection efficiency and episomal silencing.
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