The Meso Scale Discovery Phospho-S6RP (Ser240/244) Whole Cell Lysate Kit is designed to monitor phosphorylation of S6 Ribosomal Protein at Ser240/244 in cell lysates The kit includes a 96-well plate coated with capture antibody detection antibody Read Buffer Sample Diluent and lyophilized S6RP phospho-peptide standards to generate a calibration curve This electrochemiluminescent immunoassay allows sensitive detection of S6RP phosphorylation in cell lysates without the need for radioactivity The kit provides a tool for studying mTOR pathway activation and measuring effects of kinase inhibitors or other treatments on S6RP phosphorylation