Homogenizers and Immersion Blenders
Devices designed for overhead immersion into substances; ideal for pulverization of plant/animal tissue, grain/seed, sample prep, and more. Includes accessories such as generator probes of varying tips, stand assemblies, bead mill tubes, and more.
Homogenizers can be used with tissue, plant, food, soil, and other sample types. Homogenization is a common sample preparation step for analyzing nucleic acids, proteins, cells, metabolism, pathogens, and other targets.
Cell fractionation releases the contents of cells, and newer methods have been designed to consider quality and environmental aspects, cross-contamination, aerosol generations, infection risk, and noise levels. Different methods have been developed for disrupting cell walls and other structures. These include bead mills, sonication, rotor-stator mechanical, high pressure, and other physical methods.
For biological work, sonication may adequately disrupt or deactivate biological material via the process of sonoporation. Sonic dismembrators can break up cells and fragment DNA molecules by subjecting the sample to ultrasonic waves that create small fragments.
Some substances can be mechanically homogenized using beads and other small pieces of metal or minerals. The sample is added to a vial that contains these small particles; the tubes are then rapidly agitated, which breaks down the materials. Depending on the equipment, many samples can be processed simultaneously with this method.
Another mechanical homogenization method is the rotor-stator; these devices have a fast-spinning inner rotor and a stationary outer sheath (stator). Samples are subjected to tearing, shear fluid forces, and cavitation (bubble formation and collapse). A rotor-stator processes a single sample at a time; high-throughput and continuous use models are available.
An immersion blender has an electric motor that powers rotating blades at the end of a shaft. The device is lowered into the sample or mixture and the motor activated. The device may be hand-held or clamped to a stand.
Homogenizer packages are also available for motorized methods.
PREOMICS INC BEATBOX TISSUE KIT 96X
The BeatBox, cell lysis and tissue homogenization can be integrated into the PreOmics iST sample preparation workflows.
BioSpec Products Chrome-Steel Beads, 1.3mm dia, Beads are sold in one pound bottles
Chrome steel has a density of 7.9g/cc (used mostly for grinding leaves). Beads are sold in one pound bottles.Cat. No. 11079113c 1.3mm diaListed bead sizes are median (average) values. Because beads are sorted by sieves, bead size varies between +/-10% of a listed value.With either stainless steel or chrome steel beads, common polypropylene microvials will break if 'dry' grinding or cryo-grinding is attempted in our MiniBeadbeaters. In this case, our 2 ml stainless steel microvials with silicone rubber caps or our 2 ml XXTuff polypropylene screw cap microvials are recommended.
Accuflow Systems Saint-Gobain Assembly Tool, Pure-Fit SIB, 5/16 in, 312 Series
Hand tool for installing smooth inner-bore retainers and compression fittings in the 312 series. The tool is intended for manual assembly on tubing with a nominal 0.3125 in (5/16 in) inner diameter, providing repeatable retainer compression for secure fluid connections in laboratory and fluid-handling systems.
- Hand-operated assembly tool for consistent retainer compression.
- Designed for tubing with 0.3125 in (5/16 in) inner diameter.
- Compatible with 312-series smooth inner-bore retainers and fittings.
- Durable jaw profile for secure engagement during assembly.
- Suitable for manual assembly in laboratory and fluid-handling environments.