Standard PCR Reagents and Kits
Thermo Scientific™ ThermoPrime Taq DNA Polymerase, with 10X buffer and separate vial of 25 mM MgCl2
Thermo Scientific ThermoPrime Taq Taq Polymerase is a 94 kDa recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli.
Promega GoScript™ Reverse Transcription System
GoScript Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors.
Biotium EvaEZ™ Fluorometric DNA Polymerase Activity Assay (200 rxn)
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind the EvaGreen dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of the polymerase. The assay was developed for the measurement of Taq DNA polymerase activity. It can also be used for other DNA polymerases such as Pfu, Vent, Phusion, Bst, Phi29, MMLV, AMV, SuperScript, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I. The activity assay can be conducted at temperatures from 4°C to 75°C.
