Hygromycin B is an antibiotic used as a selective agent in cell culture systems, particularly for eukaryotic cell lines and microbial selection. It inhibits protein synthesis by binding to the ribosome and disrupting the elongation cycle, which leads to cell death. This liquid formulation, containing 5 vials of Hygromycin B, is commonly used in genetic research to select for cells that have been genetically modified to express resistance to this antibiotic. Hygromycin B is particularly useful in the selection of transfected mammalian cells, yeast, and bacteria, and is widely employed in gene therapy, cloning, and cellular studies.