PAN-Biotech MCDB 131 Cell Culture Medium, w/o: L-Glutamine, w: 1.176 g/L NaHCO3 (500 ml). BICARBONATE: With 1.176 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany). DESCRIPTION: MCDB 131 was developed for cultivation of human microvascular endothelial cells (HMVEC) under reduced serum content. For this purpose it is often supplemented with dialyzed serum, EGF, and hydrocortisone. MCDB 131 is suitable for other cells, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells. MCDB 131 has been used for definitive endoderm differentiation of hPSCs and as a basal medium for MSCs differentiation. MCDB 131 is formulated with several unique components, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins.