Additional Selective and Differential Cell Culture Media
NEOGEN Tryptose, 500 g
Tryptose is an enzymatic digest of protein for use in preparing microbiological culture media in a laboratory setting. Tryptose is not intended for use in the diagnosis of disease or other conditions in humans. Tryptose was developed while studying growth requirements of Brucella spp. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms including Neisseria gonorrhoeae, Streptococcus milleri and Brucella spp.. Casman reported that a medium consisting of 2% Tryptose, along with other media ingredients, equaled fresh beef infusion base with respect to organism growth. Tryptose is recommended in broth and agar formulations, and when used in media containing blood to permit hemolytic reactions. Tryptose is used in the preparation of Tryptose Broth (Product Code No. NCM0087).For more information visit https://www.neogen.com/solutions/Microbiology/tryptose
NEOGEN m-FC Agar, 500 g
m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans. Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment. Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in the fecal coliform procedure and other tests. The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.For more information visit https://www.neogen.com/solutions/Microbiology/m-fc-agar
NEOGEN Sabouraud Dextrose Broth, 5 kg
Sabouraud Dextrose Broth is used for the cultivation of fungi from sterile and non-sterile products. Sabouraud Dextrose Broth conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.A medium recommended by the Harmonized Pharmacopoeia for the enrichment of Candida albicans from non-sterile samples and fungal test strain cultivation. Conforms to USP/EP/JP performance specifications. The peptones and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating. According to the Harmonized Pharmacopoeia, Sabouraud Dextrose Broth is used as an enrichment broth, with subculture performed onto Sabouraud Dextrose Agar.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-broth
NEOGEN Tryptose Phosphate Broth, 500 g
Tryptose Phosphate Broth is used for the cultivation of a wide variety of fastidious microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. This is a versatile, nutritionally rich buffered glucose broth. The medium is a general-purpose broth that has been used as a blood culture medium and with the addition of sodium azide 0.025% as a selective medium for streptococci in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/tryptose-phosphate-broth
NEOGEN Pseudomonas Isolation Agar, 500 g
Pseudomonas Isolation Agar is used for the isolation of Pseudomonas aeruginosa and other Pseudomonas spp. in a laboratory setting. Pseudomonas Isolation Agar is not intended for use in the diagnosis of disease or other conditions in humans. Pseudomonas Isolation Agar is based on Medium A described by King, Ward, and Raney. This medium is very useful for isolating Pseudomonas spp. Pseudomonas Isolation Agar includes Irgasan®, a potent broad spectrum antimicrobial not active against Pseudomonas spp. This medium is selective and formulated to enhanced formation of blue or blue-green pyocyanin pigment by Pseudomonas aeruginosa. The pigment diffuses into the medium surrounding growth.For more information visit https://www.neogen.com/solutions/Microbiology/pseudomonas-isolation-agar
NEOGEN Phenylethanol Agar, 10 kg
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.For more information visit https://www.neogen.com/solutions/Microbiology/phenylethanol-agar
NEOGEN Schaedler Agar, 500 g
Schaedler Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Agar is not intended for use in the diagnosis of disease or other conditions in humans. Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Agar is suitable for standard procedures used in cultivating anaerobic bacteria. Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.For more information visit https://www.neogen.com/solutions/Microbiology/schaedler-agar
NEOGEN Buffered Sodium Chloride - Peptone Broth pH 7.0, 500 g
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-sodium-chloride-peptone-broth-ph-7
NEOGEN Potato Dextrose Broth, 500 g
Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting. Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth. Potato Dextrose Broth is the same formula as Potato Dextrose Agar, but agar has been omitted.For more information visit https://www.neogen.com/solutions/Microbiology/potato-dextrose-broth
NEOGEN HC Agar Base, 500 g
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.For more information visit https://www.neogen.com/solutions/Microbiology/hc-agar-base
NEOGEN Lactobacillus Selective Agar Base, 5 kg
Lactobacillus Selective Agar Base is used for the isolation and enumeration of lactobacilli in a laboratory setting. Lactobacillus Selective Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacillus Selective Agar Base was developed by Rogosa, Mitchell, and Wiseman. This medium is used for isolation, enumeration, and identification of lactobacilli in foods. The low pH and high acetate concentrations effectively suppress other bacterial flora allowing lactobacilli to flourish.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacillus-selective-agar-base
NEOGEN Lactobacillus Selective Agar Base, 500 g
Lactobacillus Selective Agar Base is used for the isolation and enumeration of lactobacilli in a laboratory setting. Lactobacillus Selective Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Lactobacillus Selective Agar Base was developed by Rogosa, Mitchell, and Wiseman. This medium is used for isolation, enumeration, and identification of lactobacilli in foods. The low pH and high acetate concentrations effectively suppress other bacterial flora allowing lactobacilli to flourish.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacillus-selective-agar-base
NEOGEN Tomato Juice Agar, 500 g
Tomato Juice Agar is used for the cultivation of lactobacilli in a laboratory setting. Tomato Juice Agar is not intended for use in the diagnosis of disease or other conditions in humans. In 1925, Mickle and Breed reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp investigated tomato juice on bacterial development and found growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White’s modification, is especially useful in cultivating L. acidophilus from foodstuffs.For more information visit https://www.neogen.com/solutions/Microbiology/tomato-juice-agar
NEOGEN SIM Medium, 500 g
SIM Medium is used for the differentiation of microorganisms on the basis of hydrogen sulfide production, indole production, and motility in a laboratory setting. SIM Medium is not intended for use in the diagnosis of disease or other conditions in humans. Semisolid media have been used extensively in the determination of bacterial motility. The production of hydrogen sulfide, indole formation, and motility are useful diagnostic tests in the identification of Enterobacteriaceae, especially Salmonella spp. and Shigella spp. In 1940, Sulkin and Willett demonstrated motility, hydrogen sulfide production, and carbohydrate fermentation by members of Salmonella and Shigella groups. They called attention to the “brush-like growth” or motility of the typhoid organisms. Greene et al. used SIM Medium to detect motility in a large series of cultures of typhoid organisms.For more information visit https://www.neogen.com/solutions/Microbiology/sim-medium
NEOGEN Terrific Broth, Modified, 500 g
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified