Additional Selective and Differential Cell Culture Media
NEOGEN Todd Hewitt Broth, 5 kg
Todd Hewitt Broth is used for the cultivation of streptococci and other fastidious microorganisms. Todd Hewitt Broth is not intended for use in the diagnosis of disease or other conditions in humans. Todd Hewitt Broth was originally developed for the production of antigenic streptococcal hemolysin. Todd Hewitt Broth is prepared according to the formula described by Updyke and Nickle, who compared media for type specific extract production of group A streptococci. This study was performed using Todd Hewitt Broth prepared with infusion of fresh beef heart as a control. Results showed Todd Hewitt Broth was particularly satisfactory for growth of group A streptococci for serological typing. Todd Hewitt Broth is recommended as an enrichment medium for growth of streptococcal cells in the identification of groups A and B by IF staining.For more information visit https://www.neogen.com/solutions/Microbiology/todd-hewitt-broth
NEOGEN DRBC Agar (BAM), 5 kg
DRBC Agar (BAM) is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans.DRBC Agar (BAM) is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-bam
Biotium CD11b / MAC-1 (Microglial Marker)(ITGAM/3337), CF647 conjugate, 0.1mg/mL
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin '2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin aM/'2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system. Primary antibodies are available purif
NEOGEN Letheen Broth with Tween, 10 kg
Letheen Broth is primarily used for assessing the bactericidal activity of quaternary ammonium compounds, and is not intended for use in the diagnosis of disease or other conditions in humans.Letheen Broth is used to determine the phenol co-efficient of cationic surfactants as recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC). It is also used in hygiene swabbing protocols where it is necessary to neutralize quaternary ammonium compounds.A modification of FDA Broth, Letheen Broth contains lecithin to neutralize quaternary ammonium compounds and Polysorbate 80 to neutralize phenols, hexachlorophene, formalin and (with lecithin) ethanol.Letheen Broth is easily prepared and has a clear appearance aiding in visual inspection for growth.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-broth-with-tween
NEOGEN Fraser Broth Base, 5 kg
Fraser Broth Base is used with Acriflavin, Nalidixic Acid, and Ferric Ammonium Citrate for the selective enrichment of Listeria spp. Fraser Broth Base is not intended for use in the diagnosis of disease or other conditions in humans.Formulated according to MLG Appendix 1.09 (for the ISO 11290 formulation please use NCM0001). Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicated the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese and pate.For more information visit https://www.neogen.com/solutions/Microbiology/fraser-broth-base
NEOGEN Letheen Agar Base Modified without Tween, 5 kg
Letheen Agar Base Modified (without Tween) is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base Modified is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-agar-base-modified-without-tween
NEOGEN Sabouraud Dextrose Agar, 10 kg
Sabouraud Dextrose Agar is used for the cultivation of fungi. Conforms to Harmonized USP/EP/JP performance requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopeia for the isolation and identification of Candida albicans from non-sterile samples. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to SDA. The medium is also used for the preparation and maintenance of fungal test strains as described by the Harmonized Pharmacopeia. The peptone digests and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating which will cause a drop in the gel strength.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar
NEOGEN Rappaport-Vassiliadis Medium with Soya (RVS) (ISO), 5 kg
Rappaport-Vassiliadis Medium with Soya is used as a selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. RVS is not intended for use in the diagnosis of disease or other conditions in humans.Rappaport et al. originally described the broth, which was later modified by Vassiliadis et al. and optimized by van Schothorst et al. Enzymatic digest of soya provides a source of nitrogen and amino acids and sodium chloride provides osmotic pressure. The potassium phosphate components act as a buffer. Magnesium chloride and malachite green act as selective agents against non-target microorganisms. The formulation is very hygroscopic and will produce a slight exothermic reaction when mixed with water.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-medium-with-soya
NEOGEN Violet Red Bile Glucose Agar (VRBGA), 10 kg
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar
NEOGEN Tryptone Soy Broth, 5 kg
Tryptone Soy Broth (TSB) is a general-purpose broth which will support the growth of a wide range of micro-organisms and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean- Casein Digest Broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-soy-broth
NEOGEN DC Medium with BCIG, 5 kg
DC Medium with BCIG is used in the chromogenic differentiation of E. coli from other coliforms in water samples using the membrane filtration method in a laboratory setting. DC Medium with BCIG is not intended for use in the diagnosis of disease or other conditions in humans.DC (Differential Coliform) Medium with BCIG is a selective and differential medium for the presumptive identification of E. coli. This medium is enhanced by the addition of a chromogenic agent, BCIG, 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide to detect glucuronidase activity. The presence of the enzyme ß-D-glucuronidase differentiates most E. coli spp. from other coliforms, and is the same enzyme used in the MUG reaction. BCIG reacts slightly differently, and when released into the medium is insoluble, accumulating within the cell, imparting a blue to purple color to presumptive E. coli colonies.For more information visit https://www.neogen.com/solutions/Microbiology/dc-medium-with-bcig
NEOGEN Palcam Agar Base, 5 kg
Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.For more information visit https://www.neogen.com/solutions/Microbiology/palcam-agar-base
NEOGEN EC Medium with MUG, 5 kg
EC Medium w/ MUG is used for the fluorogenic detection of Escherichia coli in a laboratory setting. EC Medium w/ MUG is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria is inhibited by the bile salts, while growth of E. coli is enhanced by its presence. EC Medium w/ MUG is the same formula as EC Medium, with the addition of 4-methylumbelliferyl-ß-D-glucuronide. Feng and Hartman developed a rapid assay for E. coli by incorporating 4-methylumbelliferyl-ß-D-glucuronide (MUG) into Lauryl Tryptose Broth at a final concentration of 100 ɥg/mL.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-with-mug
NEOGEN EC Broth, 10 kg
EC Broth is used for the detection of coliform bacteria at 37°C and Escherichia coli at 44.5°C. EC Broth is not intended for use in the diagnosis of disease or other conditions in humans.EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.EC Broth is made selective for coliforms by the inclusion of Bile Salts in the dehydrated medium. The selective nature of this medium ensures that the growth of non-coliform bacteria is minimized. The medium is buffered by the addition of potassium phosphates and osmotically balanced by sodium chloride. The medium is used at 37°C for coliform organisms and 44.5°C is recommended for the isolation E. coli.For more information visit https://www.neogen.com/solutions/Microbiology/ec-broth
NEOGEN Yeast Extract Agar, 10 kg
Yeast Extract Agar is used for the enumeration of microorganisms in potable and freshwater samples and is not intended for use in the diagnosis of disease or other conditions in humans. A nutrient agar corresponding to the standard formulation for the plate count of micro-organisms in water and dairy products. This medium is also useful for teaching and demonstration purposes using non-fastidious organisms.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract-agar