Additional Selective and Differential Cell Culture Media
NEOGEN UVM Broth, 500 g
This item has a minimum qty of 2 per supplier requirements.
UVM Broth is used for the selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.UVM Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food samples. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.For more information visit https://www.neogen.com/solutions/Microbiology/uvm-broth
NEOGEN Tryptic Soy Agar with Lecithin and Tween 80, 500 g
Tryptic Soy Agar with Lecithin and Tween 80 is used for the isolation of microorganisms from surfaces sanitized with quaternary ammonium compounds and is not intended for use in the diagnosis of disease or other conditions in humans. In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing “preservative carryover”. Tryptic Soy Agar with Lecithin and Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-agar-with-lecithin-and-tween-80
NEOGEN Violet Red Bile Glucose Agar (VRBGA), 500 g
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of bile-tolerant Gram-negative bacteria from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Yeast extract provides vitamins and gelatin serves as source of carbon and nitrogen. Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. The formulation is a modification of Violet Red Bile Agar by Mossel which substitutes lactose for glucose.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar
NEOGEN Universal Pre-enrichment Broth, 10 kg
Universal Pre-enrichment Broth is used for the recovery of Salmonella spp. and Listeria spp. in a laboratory setting. Universal Pre-enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. Traditional methods for recovering Salmonella spp. and Listeria spp. from food products require separate pre-enrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. Bailey and Cox formulated Universal Preenrichment Broth to permit simultaneous resuscitation of sublethally injured Salmonella and Listeria. The broth medium provides sufficient buffering capacity to prevent rapid decreases in pH and allows for repair of injured cells that might be sensitive to low pH values or inhibitory substances.For more information visit https://www.neogen.com/solutions/Microbiology/universal-preenrichment-broth
NEOGEN Middlebrook 7H11 Agar, 5 kg
Middlebrook 7H11 Agar is used with glycerol and OADC Enrichment for the cultivation of Mycobacterium spp. in a laboratory setting. Middlebrook 7H11 Agar is not intended for use in the diagnosis of disease or other conditions in humans.There are two types of solid culture media for the primary isolation of mycobacteria, coagulated egg as a base (Lowenstein formulations) and an agar base (Middlebrook formulations). The use of agar-based media for primary isolation of mycobacteria have the following significant advantages:Agar-based media do not usually liquefy in the presence of contaminating proteolytic organisms.Agar-based media retain exact concentrations of added drugs because the medium is solidified with agar rather than by inspissation of the egg. There is less drug inactivation when egg ingredients are absent.For more information visit https://www.neogen.com/solutions/Microbiology/middlebrook-7h11-agar
NEOGEN DRBC Agar (BAM), 500 g
DRBC Agar (BAM) is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans.DRBC Agar (BAM) is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-bam
NEOGEN Violet Red Bile Glucose Agar (ISO), 10 kg
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods and is not intended for use in the diagnosis of disease or other conditions in humans.Formulated according to ISO 21528:2017 (all parts), VRBGA is a modification of Violet Red Bile Lactose Agar (NCM0089) introduced by Mossel in 1978. VRBA contains lactose which is fermented by members of the coli/ aerogenes group, this medium gives a ‘coliform’ count. The VRBGA formula modification replaces lactose with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The overlay procedure ensures anaerobic conditions and suppresses the growth of non-fermentative Gram negative bacteria.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-glucose-agar-iso
NEOGEN DRBC Agar (BAM), 10 kg
DRBC Agar (BAM) is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. DRBC Agar is not intended for use in the diagnosis of disease or other conditions in humans.DRBC Agar (BAM) is based on Dichloran Rose Bengal Chlortetracycline (DRBC) Agar formula described by King, Hocking, and Pitt. DRBC Agar conforms with APHA guidelines for the mycological examination of foods containing chloramphenicol rather than chlortetracycline as proposed by King, Hocking, and Pitt. DRBC Agar is a selective medium, supporting good growth of yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-bam
NEOGEN MacConkey Agar, 10 kg
MacConkey Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements and FDA/BAM. MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Escherichia coli from non-sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of carbon and nitrogen. Lactose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. Escherichia coli can ferment lactose to produce acid which results in a pH drop. This is indicated by neutral red resulting in pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-agar
NEOGEN Baird-Parker Agar, 10 kg
Baird-Parker Agar is used for detection and enumeration of Staphylococcus aureus in foods and is not intended for use in the diagnosis of disease or other conditions in humans.Baird-Parker Agar was first described in 1962. It is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. Coagulase-positive staphylococci can grow and reproduce in cosmetic products. These products are tested for the presence of coagulase-positive staphylococci using standard microbiological methods.Formula:Enzymatic Digest of Casein - 10.0 g/LBeef Extract - 5.0 g/LYeast Extract - 1.0 g/LLithium Chloride - 5.0 g/LGlycine - 12.0 g/LSodium Pyruvate - 10.0 g/LAgar - 17.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar
NEOGEN BCYE Agar (Legionella Isolation Medium), 10 kg
BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans.In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer.For more information visit https://www.neogen.com/solutions/Microbiology/bcye-agar-legionella-isolation-medium
NEOGEN Cystine Lactose Electrolyte Deficient (CLED) Agar, 500 g
Cystine Lactose Electrolyte Deficient (CLED) Agar is used for the differentiation and enumeration of microorganisms in urine. A medium for urine culture where the absence of electrolytes inhibits the swarming of Proteus spp. Cystine is added for the benefit of those organisms which have a specific cystine requirement. Differentiation of lactose and non-lactose fermenters is achieved using bromothymol blue as pH indicator. This medium supports the growth of Streptococcus pyogenes and most other fastidious organisms that do not require blood. Formula:Balanced Peptone - 4.0 g/LBeef Extract - 3.0 g/LTryptone - 4.0 g/LLactose - 10.0 g/LL-Cystine - 0.128 g/LBromothymol Blue - 0.02 g/LAgar - 15.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/cystine-lactose-electrolyte-deficient-agar
NEOGEN Tryptone Soy Broth, 500 g
Tryptone Soy Broth (TSB) is a general-purpose broth which will support the growth of a wide range of micro-organisms and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean- Casein Digest Broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-soy-broth
NEOGEN Baird-Parker Agar, 500 g
Baird-Parker Agar is used for detection and enumeration of Staphylococcus aureus in foods and is not intended for use in the diagnosis of disease or other conditions in humans.Baird-Parker Agar was first described in 1962. It is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. Coagulase-positive staphylococci can grow and reproduce in cosmetic products. These products are tested for the presence of coagulase-positive staphylococci using standard microbiological methods.Formula:Enzymatic Digest of Casein - 10.0 g/LBeef Extract - 5.0 g/LYeast Extract - 1.0 g/LLithium Chloride - 5.0 g/LGlycine - 12.0 g/LSodium Pyruvate - 10.0 g/LAgar - 17.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar
NEOGEN Tryptone Soy Agar, 500 g
Tryptone Soy Agar (TSA) is a general-purpose agar which will support the growth of a wide range of micro-organisms, and is not intended for use in the diagnosis of disease or other conditions in humans.The medium can be used for phage typing, colicine typing and for testing the X and V factor requirements of Haemophilus spp.Formula:Tryptone - 15.0 g/LSoy Peptone - 0.5 g/LSodium Chloride - 5.0 g/LAgar - 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/tryptone-soy-agar