Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS rHTR2C β-Arrestin Cell Line, 2 vials. (93-0915C3)
The PathHunter rHTR2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of rHTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rHTR2C and EA-tagged β-Arrestin-2. Activation of rHTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter VIPR1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0739C3)
The PathHunter VIPR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of VIPR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged VIPR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting VIPR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter EDG5 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0735C3)
The PathHunter EDG5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of EDG5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged EDG5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting EDG5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter DLD1 IL7R/CRLF2 Dimerization Cell Line, 2 vials. (93-1019C13)
The PathHunter IL7R/CRLF2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. TSLP) induced dimerization of IL7R/CRLF2 receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside CRLF2 target protein fused to EA. Upon ligand-induced dimerization of IL7R/CRLF2, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Eurofins Discoverx PathHunter U2OS PRLR-JAK1 Functional Assay, 2 vials. (93-0686C3)
The PathHunter PRLR-JAK1 Functional assay cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting CTKs. This cell line enables assessment of PRLR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PRLR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter PROKR1 Total GPCR Internalization U2OS Cell Line, 2 vials. (93-0764C3)
The PathHunter PROKR1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of PROKR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PROKR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PROKR1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 rCHRM4 β-Arrestin Cell Line, 2 vials. (93-0870C2)
The PathHunter rCHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 OXER1 β-Arrestin Cell Line, 2 vials. (93-0867C2)
The PathHunter OXER1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. 5-oxo-ETE) based activation of OXER1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXER1 and EA-tagged β-Arrestin-2. Activation of OXER1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS ErbB1 Functional Assay, 2 vials. (93-0863C3)
The PathHunter U2OS ErbB1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated ErbB1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged ErbB1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter ADRB2 Total Internalization HEK 293 Cell Line, 2 vials. (93-0800C1)
The PathHunter ADRB2 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of ADRB2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged ADRB2 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADRB2 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS CSF1R/CSF1R Dimerization Cell Line, 2 vials. (93-1061C3)
The PathHunter CSF1R/CSF1R Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter CHO-K1 BAI2 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0546C2)
The PathHunter BAI2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of BAI2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI2 and EA-tagged β-Arrestin-2. Activation of BAI2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS mCCR2 β-Arrestin Cell Line, 2 vials. (93-0543C3)
The PathHunter mCCR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL2/MCP-1) based activation of mCCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR2 and EA-tagged β-Arrestin-2. Activation of mCCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 rPROKR1 β-Arrestin Cell Line, 2 vials. (93-0544C2)
The PathHunter rPROKR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prokineticin) based activation of rPROKR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rPROKR1 and EA-tagged β-Arrestin-2. Activation of rPROKR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 BAI1 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0545C2)
The PathHunter BAI1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of BAI1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI1 and EA-tagged β-Arrestin-2. Activation of BAI1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.