Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/H2/G15 Stable Cell Lin
Histamine is a ubiquitous messenger molecule released from mast cells enterochromaffin-like cells and neurons Its various actions are mediated by histamine receptors H1 H2 H3 and H4 Specifically the histamine receptor H2 belongs to the rhodopsin-like family of G protein-coupled receptors As an integral membrane protein it stimulates gastric acid secretion and regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation
iQ Biosciences Huacaya Alpaca PBMCs, 50M cells/vial
High-quality, high-viability (> 80%) huacaya alpaca peripheral blood mononuclear cells (PBMCs) sourced responsibly from a vetted third party facility. Potential uses include, production of stable single-domain antibodies (nanobodies) for therapeutic and diagnostic applications, vaccine development, and autoimmune research. All orders come with an iQ Certificate of Analysis.
Genscript Corporation CHO-K1/NPY2/Gqi5 Stable Cell L
The NPY family consists of three 36-amino acid peptides neuropeptide Y (NPY) peptide YY (PYY) and pancreatic polypeptide (PP) which bind to the NPY receptors At present five distinct NPY receptors Y1 Y2 Y4 Y5 and y6 have been established by receptor cloning studies and all of them are G sub i /sub -coupled GPCRs Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake inhibition of anxiety in the CNS presynaptic inhibition of neurotransmitter release in the CNS and periphery modulation of circadian rhythm release of pituitary hormones modulation of hippocampal activity pain transmission vasoconstriction inhibition of insulin release and modulation of renal function With regard to endogenous agonists the receptors Y2 preferentially bind NPY and PYY
Eurofins Discoverx cAMP Hunter CHO-K1 rDRD2S (Short Isoform) Gi Cell Line, 2 vials. (95-0129C2)
The cAMP Hunter rDRD2S Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine, pergolide) based activation of rDRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type rDRD2S, a naturally Gi/Go coupled GPCR. Upon activation of rDRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Abcam CGP 35348, GABAB antagonist, 10MG
MW 225.22 Da. Selective GABAB antagonist (IC50 = 34 μM). Brain penetrant *in vivo*. Also available in simple stock solutions (ab146707) - add 1 ml of water to get an exact, ready-to-use concentration.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter CHO-K1 GPR92 β-Arrestin Cell Line, 2 vials. (93-0372C2)
The PathHunter GPR92 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPAs) based activation of GPR92 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR92 and EA-tagged β-Arrestin-2. Activation of GPR92 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR78 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0370C2A)
The PathHunter GPR78 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR78 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR78 and EA-tagged β-Arrestin-2. Activation of GPR78 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR143 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0362C2A)
The PathHunter GPR143 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR143 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR143 and EA-tagged β-Arrestin-2. Activation of GPR143 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS FGFR1-α-Klotho Functional Assay, 2 vials. (93-1117C3)
The PathHunter U2OS FGFR1-α-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR1 and α-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR1 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, α-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter Jurkat PD-1 Signaling Assay (SHP-2), 2 vials. (93-1106C19)
The PathHunter PD-1 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. PD-L1, PD-L2) based activation of PD-1 receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PD-1 target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.