Cell Lines and Cell Cultures
ATCC Escherichia coli; 6 x 0.3mL
ATCC 35218-MINI-PACK™ consists of 6 ready-to-use vials of ATCC 35218™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
ATCC Proteus penneri; Strain CDC 1808-73 N
Proteus penneri strain CDC 1808-73 was isolated in Anchorage, Alaska, from a urine sample. This bacterial type strain grows aerobically in nutrient agar/broth.
ATCC PBAD18KAN PLASMID IN E COLI
One of several tightly controlled expression vectors (ATCC 87393 - ATCC 87402) regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning sites and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Plasmid contains bla (ampR) sequences surrounding the cmLR gene which could promote recombination if this plasmid is used in combination with other compatible ampR plasmids. Recombination can be avoided by the use of recA host strains, or it can be used to advantage to intentionally exchange markers among plasmids. The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp
ATCC Clostridioides difficile; Strain 4206; genomic DNA
Genomic DNA isolated from Clostridioides difficile Strain 4206. This bacterial strain is also available as ATCC Catalog No. BAA-1872™.
ATCC Enterococcus faecium
This whole-genome sequenced strain of Enterococcus faecium was isolated from human feces. This strain is resistant to vancomycin and teicoplanin and was confirmed by PCR to carry the vanA gene. It has applications in antimicrobial resistance research and drug development research.
ATCC HCM-CSHL-0089-C25; Cancer Model Primary; Pancreatic Ductal Adenocarcinoma; Human (Homo sapiens)
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Eurofins Discoverx ChemiScreen MCH2 Melanin-concentrating Hormone Receptor Stable Cell Line, 2 vials. (HTS026C)
The ChemiScreen MCH2 Melanin-concentrating Hormone Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MCHR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay CCR10 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS014RTA)
The Ready-to-Assay CCR10 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. fractalkine) based activation of CCR10 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR10 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR10, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CRF1 Receptor Membrane Preparation, 1 vial. (HTS023M)
The ChemiScreen CRF1 Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CRHR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Escherichia coli; 6 x 0.3mL
Escherichia coli strain AMC 198 (ATCC 11229-MINI-PACK) consists of 6 ready-to-use vials of ATCC 11229 frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping. This product has a variety of applications including testing membrane filters, absorbant pads, antimicrobial handwashing formulations, disinfectants, ink grids, and sanitizers.
ATCC Rotavirus A; Strain: Hu/Australia/1-9-12/77/S
Rotavirus A strain Hu/Australia/1-9-12/77/S was isolated in 1977 from human feces in Melbourne, Australia. This strain is whole-genome sequenced and has applications in enteric disease research.
ATCC Staphylococcus aureus; subsp. aureus; genomic DNA
Genomic DNA isolated from Staphylococcus aureus subsp. aureus strain Wood 46. This bacterial strain is also available as ATCC® 10832™.
ATCC Escherichia coli
Escherichia coli strain Crooks was isolated from feces. This certified reference material can be used in testing and calibration in ISO 17025-accredited laboratories to challenge assay performance, validate or compare test methods, and establish sensitivity, linearity, and specificity during assay validation or implementation.
ATCC Human herpesvirus 3; (attenuated VZV); Strain: Webster
Human herpesvirus 3 strain Webster is propagated in MRC-5. This attenuated strain was isolated from a human patient and was deposited by SA Plotkin.
ATCC Saccharopolyspora erythraea; M5-12259; genomic DNA
Genomic DNA isolated from Saccharopolyspora erythraea strain M5-12259. This bacterial strain is also available as ATCC Catalog No. 11635™.