General Cell Lines and Cell Cultures
Eurofins Discoverx cAMP Hunter CHO-K1 CALCRL-RAMP3 Gs Cell Line, 2 vials. (95-0169C2)
The cAMP Hunter CALCRL-RAMP3 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP3. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. This product expresses CALCRL alongside the receptor modifying protein RAMP3, which increases activity of the CALCRL receptor For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Mycoplasma pulmonis
Mycoplasma pulmonis strain [Ash, PG34; L3] is a bacterial type strain that was isolated from the lung lesion of a rat. Growth of this strain is best achieved if broth cultures are allowed to incubate aerobically and the plates in 5% CO2.
ATCC Bacillus pacificus
Bacillus pacificus strain NRS 248 is a xylose-positive variant. This bacterium is whole-genome sequenced and is a microbiome standard component.
ATCC SHM-D33; Somatic Cell Hybrid; Human (Homo sapiens)/Mouse (Mus musculus)
SHM-D33 is a somatic B lymphoblast cell hybridoma that was isolated from the heteromyeloma of a mouse. This cell line was deposited by HS Kaplan and can be used in immunology research.
Eurofins Discoverx cAMP Hunter 1321N1 CALCR-RAMP3 Gs Cell Line, 2 vials. (95-0166C6)
The cAMP Hunter CALCR-RAMP3 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCR, a naturally Gs coupled GPCR, alongside modulator protein RAMP3. Upon activation of CALCR-RAMP3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. This product expresses CALCR alongside the receptor modifying protein RAMP3, which increases activity of the CALCR receptor For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 GABBR1-GABBR2 Gi Cell Line, 2 vials. (95-0165C2)
The cAMP Hunter GABBR1-GABBR2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GABA) based activation of GABBR1/GABBR2 GPCR heterodimer activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GABBR1, a naturally Gi/Go coupled GPCR, alongside its partner subunit GABBR2. Upon activation of GABBR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Chromobacterium subtsugae
Chromobacterium subtsugae strain SC 11,378 is a bacterium cited to produces beta-lactam antibiotics EM5117 and SQ 26,180 and beta-lactam potentiators SQ 28,504 and SQ 28,546.
Eurofins Discoverx Ready-to-Assay C3a Anaphylotoxin Receptor Frozen Cells, 2 Vials of Cells. (HTS016RTA)
The Ready-to-Assay C3a Anaphylotoxin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. C3a) based activation of C3AR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses C3AR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of C3AR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GAL1 Galanin Receptor Frozen Cells, 2 Vials of Cells. (HTS094RTA)
The Ready-to-Assay GAL1 Galanin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GALR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GALR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay CXCR4 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS004RTA)
The Ready-to-Assay CXCR4 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR4 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR4, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Lactobacillus rhamnosus; genomic DNA
Genomic DNA isolated from Lactobacillus rhamnosus. This bacterial strain is also available as ATCC Catalog No. 21052™.
ATCC Staphylococcus aureus; 6 x 0.3mL
ATCC BAA-1026-MINI-PACK™ consists of 6 ready-to-use vials of ATCC BAA-1026™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Lactobacillus delbrueckii subsp. delbrueckii strain 730 (ATCC 9649™)
Lactobacillus delbrueckii subsp. delbrueckii strain 730 (ATCC 9649™) is a whole-genome sequenced type strain isolated from sour grain mash. It produces D(-)lactic acid and is used in food production research and bioinformatics.
Recommended companion product:
Quantitative Genomic DNA (Fisher Cat. No. 50-238-3915, ATCC 9649DQ™), ideal for qPCR assay development and microbial load quantification.
ATCC Lactococcus lactis; subsp. lactis; genomic DNA
Genomic DNA isolated from Lactococcus lactis subsp. lactis strain NCTC 6681. This bacterial strain is also available as ATCC Catalog No. 19435™.