General Cell Lines and Cell Cultures
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Neurons (SCN)
This is a neural cell specific Basic Nucleofector Kit which allows to determine the optimal transfection conditions for any mammalian neuron type. In contrast to our standard Basic Kit (VPI-1003) this kit is suited for transfection of small cell numbers.IMPORTANT NOTE: It only works in combination with an older Nucleofector Device model, the Nucleofector IIS Device.Benefits:Efficient transfection of neural or glial cells in small scale (20 µL)Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Applications:Transfection of low cell numbers in the range of 2 - 5 x10⁴ cellsKit suited for various neuron types from different mammalian species
iXCells Biotechnologies Peripheral Blood Mononuclear Cells (PBMCs), 25M
Peripheral Blood Mononuclear Cells (PBMCs) is any blood cell with a single nucleus. PBMCs consist of lymphocytes (T, B and NK cells), monocytes and dendritic cells. iXCells Biotechnologies offers PBMCs from the human peripheral blood of individual healthy donors. These PBMCs are purified from peripheral blood using ficoll gradient centrifugation.
Rockland Immunochemicals CHICKEN RED BLOOD CELLS (RBC) 10% Washed Pooled Cells - R401-0100
Chicken whole blood is washed to remove the platelet rich plasma, buffy coat layer, and leukocytes (WBC). Red blood cells are supplied as a 10 percent suspension in phosphate buffered saline (PBS). Chicken red blood cells are useful for the titration of complement, adsorption procedures, testing for agglutinins/HA assays, and for the preparation of stroma as particulate reagents. Chicken red blood cells are perishable and are collected and processed upon receipt of your order.
Enzo Life Sciences LEADING LIGHT Wnt Reporter Cell Line (1 ml)
Wnt reporter cell line for discovering Wnt pathway modulators. The LEADING LIGHT. .The assay has been used successfully in different assay formats (including HTS applications) to identify several distinct categories of small molecule compounds that modulate the Wnt signaling pathway..Note: the complete LEADING LIGHT Wnt Reporter Assay Starter Kit (Prod. No. ENZ-61001) is available. Alternative name: Wnt reporter cell line. Shipping: Shipped on Dry Ice. Long Term Storage: -80°C
ATCC C2C12; Muscle Myoblast; Mouse (Mus musculus)
C2C12 is a myoblast cell line that is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.
ATCC's L-Glutamine, 200 mM, 100X
L-Glutamine 200 mM Solution (29.23 mg/mL). L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH. All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of ‘spiking’ the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added. It has applications for cell culture, cell growth, and viability.
Human respiratory syncytial virus strain A2 (ATCC VR-1540™)
Human respiratory syncytial virus strain A2 (ATCC VR-1540™) is a whole-genome sequenced virus isolated from the lower respiratory tract of an infant with bronchiolitis and bronchopneumonia in Melbourne, Australia. Used in respiratory disease research and molecular assay development. Recommended companion products: Genomic RNA (Fisher Cat. No. 50-238-2423, ATCC VR-1540DQ™), HEp-2 cells (Fisher Cat. No. 50-238-5019, ATCC CCL-23™), EMEM (Fisher Cat. No. 50-238-2632, ATCC 30-2003™), and Fetal bovine serum (Fisher Cat. No. 50-238-3771, ATCC 30-2020™).
ATCC NCI-H1915; Lung Carcinoma; Human (Homo sapiens)
NCI-H1915 [H1915] are large cells isolated from the lungs of a 61-year-old, White female patient with stage 4 non-small cell lung cancer: carcinoma. This product has applications in cancer research and neuroscience.
ATCC FaDu; Pharyngeal Carcinoma; Human (Homo sapiens)
FaDu is a cell line with epithelial morphology that was established in 1968 from a punch biopsy of a hypopharyngeal tumor removed from a 56-year-old, White, male patient with squamous cell carcinoma. This cell line is a suitable transfection host and has applications in cancer and immuno-oncology research.