General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 rEDG5 β-Arrestin Cell Line, 2 vials. (93-0582C2)
The PathHunter rEDG5 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of rEDG5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rEDG5 and EA-tagged β-Arrestin-2. Activation of rEDG5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 rCRTH2 β-Arrestin Cell Line, 2 vials. (93-0580C2)
The PathHunter rCRTH2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin D2) based activation of rCRTH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCRTH2 and EA-tagged β-Arrestin-2. Activation of rCRTH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mSSTR5 β-Arrestin Cell Line, 2 vials. (93-0627C2)
The PathHunter mSSTR5 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. somatostatin 28) based activation of mSSTR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mSSTR5 and EA-tagged β-Arrestin-2. Activation of mSSTR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS AVPR1A β-Arrestin Cell Line, 2 vials. (93-0587C3)
The PathHunter AVPR1A cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 sEDG5 β-Arrestin Cell Line, 2 vials. (93-0622C2)
The PathHunter sEDG5 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of sEDG5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged sEDG5 and EA-tagged β-Arrestin-2. Activation of sEDG5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Simian version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS CD137 Signaling Assay, 2 vials. (93-1089C3)
The PathHunter CD137 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of CD137 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged CD137. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PathHunter U2OS FcγRIIa Clustering Cell Line, 2 vials. (93-1140C3)
The Path Hunter FcγRIIa clustering cell line is a stable, engineered cell line designed to increase signal produced from a Checkpoint Cell Line via antibody-mediated crosslinking of the receptors. This product is a companion product used to determine cross-linking based increases in potency of agonist antibodies. The cell line included overexpresses FcγRIIa Fcg receptor. When co-cultured with a Checkpoint Cell Line in the presence of agonist antibody, the Fcg receptor binds to the antibody, inducing receptor clustering. The induced receptor clustering leads to increased activation and downstream signaling, leading to increased EFC signal production. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Promocell GmbH PromoCell hMDM-MCSF(-)-c 5 million Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, cryopreserved
PromoCell's crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in PromoCell's well-proven M2 Macrophage Generation Media XF and are available as non-activated, fully qualified M2- (M-CSF-) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells's proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.Our M2 Macrophages are now also available from HLA-typed donors.
SAMSARA SCIENCES HUMAN HEPATIC STELLATE CELLS
cryopreserved human hepatic stellate cells at p0; 1.5Mcells/vial
Medchemexpress LLC CD47 protein, rat (HEK293, His) | >95.0% | 10 UG
Recombinant rat CD47 extracellular domain expressed in HEK293 cells with a C-terminal His tag. Supplied lyophilized and intended for research use; the protein is highly pure and shows heterogeneous migration by SDS-PAGE due to glycosylation.
- Recombinant extracellular domain (Q19-K140) with C-terminal His tag.
- High purity (>95%) as determined by reducing SDS-PAGE.
- Apparent molecular weight 23-40 kDa because of glycosylation.
- Lyophilized formulation from 0.22 μm filtered PBS, pH 7.4.
- Recommended reconstitution at ≥100 μg/mL in ddH2O; aliquot and store at -20°C or -80°C for long term.
- Suitable for immunoassays, binding studies, and antibody characterization.
Eurofins Discoverx PathHunter CHO-K1 ADRA2C β-Arrestin Cell line, 2 vials. (93-0511C2)
The PathHunter ADRA2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA2C and EA-tagged β-Arrestin-2. Activation of ADRA2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mEDG1 β-Arrestin Cell Line, 2 vials. (93-0476C2)
The PathHunter mEDG1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. S1P) based activation of mEDG1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDG1 and EA-tagged β-Arrestin-2. Activation of mEDG1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 EDG7 β-Arrestin Cell Line, 2 vials. (93-0636C2)
The PathHunter EDG7 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPA) based activation of EDG7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG7 and EA-tagged β-Arrestin-2. Activation of EDG7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunterNR Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0737C3)
The PathHunter MLNR Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of MLNR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged MLNR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting MLNR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHRM3 Total Internalization U2OS Cell Line, 2 vials. (93-0775C3)
The PathHunter CHRM3 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CHRM3 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CHRM3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM3 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.