General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay GLP-1 Receptor Frozen Cells, 2 Vials of Cells. (HTS163LRTA)
The Ready-to-Assay GLP-1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-1) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen TSH Glycoprotein Hormone Receptor Stable Cell Line, 2 vials. (HTS133C)
The ChemiScreen TSH Glycoprotein Hormone Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. TSH) based activation of TSHR (L) GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TSHR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TSHR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CB1 Cannabinoid Receptor Stable Cell Line, 2 vials. (HTS019C)
The ChemiScreen CB1 Cannabinoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. cannabinoids, CP 55,940) based activation of CNR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CNR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CNR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen GABAB Receptor Membrane Preparation, 1 vial. (HTS119M)
The ChemiScreen GABAB Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of GABBR1 - GABBR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen M5 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS075M)
The ChemiScreen M5 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PrecisION hKv2.1/hKv9.2 Recombinant Stable Cell Line, 2 vials. (CYL3067)
The PrecisION hKv2.1/hKv9.2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv2.1/Kv9.2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv2.1/Kv9.2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx ChemiScreen EP4 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS142C)
The ChemiScreen EP4 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen Y2 Neuoropeptide Y Receptor Stable Cell Line, 2 vials. (HTS066C)
The ChemiScreen Y2 Neuropeptide Y Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NPY2R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NPY2R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099RTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PrecisION hGABAA α6/β3/γ2 Recombinant Stable Cell Line, 2 vials. (CYL3086)
The PrecisION hGABAA α6/β3/γ2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying GABAA α6/β3/γ2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses GABAA α6/β3/γ2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PrecisION hKv7.4/hKv7.5 Recombinant Stable Cell Line, 2 vials. (CYL3096)
The PrecisION hKv7.4/hKv7.5 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv7.4/Kv7.5 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv7.4/Kv7.5 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx ChemiScreen Motilin Receptor Membrane Preparation, 1 vial. (HTS121M)
The ChemiScreen Motilin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MLNR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen BB1 Bombesine/Neuromedin B Receptor Stable Cell Line, 2 vials. (HTS123C)
The ChemiScreen BB1 Bombesin/Neuromedin B Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bombesin, neuromedin B) based activation of NMBR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NMBR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NMBR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells, 2 Vials of Cells. (HTS135RTA)
The Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.