General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 GPR149 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0420C2A)
The PathHunter GPR149 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR149 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR149 and EA-tagged β-Arrestin-2. Activation of GPR149 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Providencia rettgeri; Strain 1103204
Providencia rettgeri strain bMx# 1103204 is a whole-genome sequenced clinical isolate. The depositor states that this bacterium is a carbapenemase producer (OXA-48).
Invivogen 293-DUAL HSTING-R232 CELLS 3-7
Dual IRF and IFN-β reporter 293 cells expressing R232 isoform of human STING, 3-7 x 10e6 cells
Eurofins Discoverx PathHunter HEK 293 CCR7 β-Arrestin Cell Line, 2 vials. (93-0195C1)
The PathHunter CCR7 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR7 and EA-tagged β-Arrestin-2. Activation of CCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mEDNRB β-Arrestin Cell Line, 2 vials. (93-0602C2)
The PathHunter mEDNRB cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. endothelin-3) based activation of mEDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRB and EA-tagged β-Arrestin-2. Activation of mEDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 PPARγ Protein Interaction Cell Line, 2 vials. (93-0390C2)
The PathHunter PPARγ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. prostaglandin J2, fatty acids) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged PPARγ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PPARγ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Lonza Walkersville 4D-Nucleofector Automat Pack
The 4D-Nucleofector Automation Package provides the interface that is required for using the 4D-Nucleofector 96-well Unit in a liquid handling system for automated medium throughput Nucleofection It also includes an interface documentation and potential support for integration
Lonza Walkersville Mouse C57BL/6 Hepatocytes Cryo
All our suspension cryopreserved primary animal hepatocytes are specifically pre-tested for metabolic activity of general Phase I and Phase II metabolism in suspension They are not suitable for applications requiring adherant cells
Lonza Walkersville NZ White Hepatocytes Suspens
All our suspension cryopreserved primary animal hepatocytes are specifically pre tested for metabolic activity of general Phase I and Phase II metabolism in suspension They are not suitable for applications requiring adherant cells
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099RTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS092LRTA)
The Ready-to-Assay EP3 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Quantitative Synthetic; Chlamydia trachomatis; LGV Type 1 DNA
Quantitative Synthetic Chlamydia trachomatis LGV Type 1 DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load. Preparation includes fragments from MOMP, 16S rRNA, pmpH, dnaB, putative virulence plasmid integrase regions, and conserved hypothetical virulence plasmid protein.
ATCC PK(15); Kidney; Pig (Sus scrofa)
PK(15) [PK15, PK-15] is a cell line exhibiting epithelial morphology that was isolated from the kidney of an adult pig.
Eurofins Discoverx PathHunter Rat CNR2 (CB2) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Rat CNR2 (CB2) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure rCNR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Biotium CD8A (Cytotoxic- & Suppressor T-Cell Marker) (C8/1779R), CF647 conjugate, 0.1mg/mL
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note:
