General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter DLD1 mPTGER4 β-Arrestin Cell Line, 2 vials. (93-0982C13)
The PathHunter mPTGER4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of mPTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTGER4 and EA-tagged β-Arrestin-2. Activation of mPTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Lonza Walkersville Hu Melanocyte - Neo Nufe Kit
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits each of them dedicated to an individual primary cell This Nucleofector Kit is the optimal kit for efficient transfectiion of primary human melanocytes The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Eurofins Discoverx PathHunter U2OS IL5RA/CSF2RB Dimerization Cell Line, 2 vials. (93-0972C3)
The PathHunter IL5RA/CSF2RB Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-5) induced dimerization of IL5RA/CSF2RB receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside IL5RA target protein fused to EA. Upon ligand-induced dimerization of IL5RA/CSF2RB, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Eurofins Discoverx PathHunter GPR35 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0968C3)
The PathHunter GPR35 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS CALCR (No RAMP) β-Arrestin Cell Line, 2 vials. (93-0566C3)
The PathHunter CALCR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Discovery Life Sciences Breast Ca TNBC Act Tx IO PBMC
PBMCs Peripheral Blood Mononuclear Cells are immune cells isolated from whole blood and viably cryopreserved providing a foundational material for immunology studies cell therapy research and functional immune assays
Eurofins Discoverx cAMP Hunter CHO-K1 PROKR1 Gs Cell Line, 2 vials. (95-0090C2)
The cAMP Hunter PROKR1 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PROKR1, a naturally Gs coupled GPCR. Upon activation of PROKR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Revvity Health Sciences Inc AequoZen Human Muscarinic M1 Cell Line, CHO-K1 Frozen Cells
AequoZen Human Muscarinic M1 Cell Line, CHO-K1 Frozen Cells
Revvity Health Sciences Inc cAMPZen Frozen Human Adrenergic α2A Cell Line, CHO-K1 Cells
cAMPZen Frozen Human Adrenergic α2A Cell Line, CHO-K1 Cells
Eurofins Discoverx PathHunter CXCR1 (IL8RA) Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0659C3)
The PathHunter CXCR1 (IL8RA) Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CXCR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR1 (IL8RA). Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR1 (IL8RA)-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Discovery Life Sciences Gastric Ca Tx Naive PBMC
PBMCs Peripheral Blood Mononuclear Cells are immune cells isolated from whole blood and viably cryopreserved providing a foundational material for immunology studies cell therapy research and functional immune assays
Eurofins Discoverx PathHunter CHO-K1 PPARδ Protein Interaction Cell Line, 2 vials. (93-0492C2)
The PathHunter PPARδ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. fatty acids, L-165041) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged PPARδ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PPARδ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.