General Cell Lines and Cell Cultures
ATCC Escherichia coli; Strain 1101362
Escherichia coli strain 1101362 is a drug-resistant bacterium that was confirmed by PCR to contain the blaKPC gene. This strain demonstrates resistance to imipenem and ertapenem and it can be used in antimicrobial resistance research and multidrug testing.
ATCC NCI-H2171; Lung Carcinoma; Human (Homo sapiens)
NCI-H2171 [H2171] are lymphoblasts exhibiting floating clusters morphology that was isolated from the lungs of a 50-year-old, White male patient with stage E small cell lung cancer: carcinoma. This product has applications in immunology and cancer research.
ATCC Escherichia coli; 6 x 0.3mL
ATCC 25922-MINI-PACK™ consists of 6 ready-to-use vials of ATCC 25922™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Eurofins Discoverx PathHunter CHO-K1 mEBI2 β-Arrestin Cell Line, 2 vials. (93-0953C2)
The PathHunter mEBI2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dihydroxycholesterol) based activation of mEBI2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEBI2 and EA-tagged β-Arrestin-2. Activation of mEBI2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC C3A (HepG2/C3A); Hepatocellular Carcinoma; Human (Homo sapiens)
C3A [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)] is an epithelial-like cell isolated from the liver of a White, 15-year-old male patient with hepatocellular carcinoma. This cell line was deposited by Baylor College of Medicine and used in cancer, toxicology, and immuno-oncology research. This product is an ATCC manufactured and accessioned progeny of ATCC CRL-10741 cited in US Pat. No. 5,290,684.
ATCC Aedes albopictus; clone C6/36; Whole Larva; Asian Tiger Mosquito (Aedes albopictus)
Aedes albopictus clone C6/36 is a cell that was isolated from the larva of an Asian tiger mosquito. This cell line was deposited by KH Eckels.
ATCC NALM6, clone G5; Lymphoma; Human (Homo sapiens)
NALM6, clone G5 is a lymphocyte-like that was isolated from the peripheral blood of a 19-year-old, male patient with acute lymphoblastic leukemia. This cell line was deposited by RS (Treichel) Salter (Oberlin College) in 2010.
Eurofins Discoverx PathHunter CHO-K1 CCRL1 β-Arrestin Cell Line, 2 vials. (93-0946C2)
The PathHunter CCRL1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL19/MIP-3β) based activation of CCRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCRL1 and EA-tagged β-Arrestin-2. Activation of CCRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 PPYR1 (NPY4R) Gi Cell Line, 2 vials. (95-0087C2)
The cAMP Hunter PPYR1 (NPY4R) Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. pancreatic polypeptide, peptide YY) based activation of PPYR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PPYR1, a naturally Gi/Go coupled GPCR. Upon activation of PPYR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CXCR4 Total Internalization U2OS Cell Line, 2 vials. (93-0890C3)
The PathHunter CXCR4 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CXCR4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CXCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CXCR4 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Promocell GmbH PromoCell NHEK.f-c 500,000 Human Epidermal Keratinocytes, derived from foreskin, single donor, cryopreserved
Primary Normal Human Epidermal Keratinocytes (NHEK) are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells.Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.
ATCC SK-MEL-24; Malignant Melanoma; Human (Homo sapiens)
SK-MEL-24 is a cell line exhibiting stellate morphology that was isolated from the skin of a 67-year-old, White male patient with melanoma. This product has applications in cancer research and immuno-oncology.
ATCC Clostridioides difficile
Clostridioides difficile strain VPI 10463 was isolated from an abdominal wound. This strain is toxigenic (toxinotype 0) and was confirmed by PCR to contain the tcdA and tcdB genes. The whole-genome sequenced bacterial strain has applications in enteric disease research and infectious disease research.
ATCC SIMA9 BRAIN MICROGLIA MOUSE MU
SIM-A9 is a microglial cell line that was derived from cerebral cortex tissues collected and pooled from mouse pups. The tissues were dissociated by trypsinization and plated. On day 14 the microglial cells were harvested by vigorously shaking the flasks and the plating out detached microglial cells. The plates were maintained for 2 weeks at which time an unexpected, extensive proliferation of microglial cells was observed. The cells were passaged 7 times over a 4-week period and were determined to have become spontaneously immortalized. The cells were then plated out in a limited dilution procedure and colonies derived from wells observed to contain a single cell were expanded. A clone designated as “A-9” gave rise to this cell line. These spontaneously immortalized micgoglial cells exhibit phagocytic activity upon liposaccharide stimulation and TNFα cytokine secretion upon β-amyloid exposure. These cells may be used in place of primary microglial cultures, whic
Eurofins Discoverx Ready-to-Assay APJ Apelin Receptor Frozen Cells, 2 Vials of Cells. (HTS068RTA)
The Ready-to-Assay APJ Apelin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. apelin) based activation of AGTRL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTRL1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AGTRL1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.