General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation, 1 vial. (HTS057M)
The ChemiScreen PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of PRLHR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Mycoplasma felis
This strain of Mycoplasma felis was isolated from the eye of a cat with conjunctivitis. This culture is a bacterial type strain and is propagated in a 5% CO2 atmosphere on Mycoplasma Medium.
Eurofins Discoverx ChemiScreen CXCR2 Chemokine Receptor Stable Cell Line, 2 vials. (HTS002C)
The ChemiScreen CXCR2 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen Motilin Receptor Stable Cell Line, 2 vials. (HTS121C)
The ChemiScreen Motilin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MLNR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MLNR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay PK1 Prokinecticin Receptor Frozen Cells, 2 Vials of Cells. (HTS074RTA)
The Ready-to-Assay PK1 Prokinecticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PROKR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Campylobacter jejuni; subsp. doylei
Campylobacter jejuni subsp. doylei strain NCTC 11951 is a bacterial type strain that was isolated in Australia from human feces. This strain has applications in enteric disease research.
Eurofins Discoverx ChemiScreen NK2 Tachykinin Receptor Stable Cell Line, 2 vials. (HTS144C)
The ChemiScreen NK2 Tachykinin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. tachykinins) based activation of TACR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TACR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TACR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay α1B Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS158RTA)
The Ready-to-Assay α1B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiBrite α2B Adrenergic Receptor Stable Cell Line, 2 vials. (HTS157L)
The ChemiBrite α2B Adrenergic Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA2B alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADRA2B, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay MC5 Melanocortin Receptor Frozen Cells, 2 Vials of Cells. (HTS155RTA)
The Ready-to-Assay MC5 Melanocortin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MC5R alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MC5R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Staphylococcus aureus; 6 x 0.3mL
ATCC BAA-976-MINI-PACK™ consists of 6 ready-to-use vials of ATCC BAA-976™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
ATCC Salmonella enterica; subsp. enterica; serovar Typhimurium
Salmonella enterica subsp. enterica serovar Typhimurium strain WG49 is a whole-genome sequenced bacterium with applications in enteric disease research. This strain is a male-specific coliphage host.