Mouse Secondary Antibodies
Biotium MSH6 (DNA Mismatch Repair Protein)(MSH6/3086), CF568 conjugate, 0.1mg/mL
The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targ
Biotium Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)(KRT15/2957), CF568 conjugate, 0.1mg/mL
Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for
Biotium Desmoglein-1 (DSG1)(32-2B), CF740 conjugate, 0.1mg/mL
Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF
Biotium Spectrin Alpha 1 (Erythrocyte Marker) (SPTA1/2939R), Biotin conjugate, 0.1mg/mL
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous alpha and beta chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin alpha I and spectrin beta I are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta II (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dye
Biotium Prolactin (Pituitary Tumor Marker)(PRL/2910), CF740 conjugate, 0.1mg/mL
Prolactin is a growth factor that is secreted by the anterior pituitary. It is necessary for the proliferation and differentiation of the mammary glands. Prolactin is useful in the classification of pituitary tumors and study of pituitary disease. It also plays a role in the development of mammary cancer, functioning dually as a mitogen and a differentiating agent. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium NK1.1 / CD161c / Klrb1c, Mouse(PK136), 0.2mg/mL
NK1. 1 is a type II integral membrane glycoprotein with a C-type lectin domain and is encoded by theKlrb1c/NKR-P1Cgene. It is predominantly expressed as a disulfide-linked homodimer on NK cells however; it is also expressed on NK-T cells, a rare population of T lymphocytes. NK1. 1 is expressed in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allograftsin vivo. NK-1. 1 has also been shown to play a role in NK cell activation, IFN- production, and cytotoxic granule release. NK-1. 1 is commonly used as a mouse NK cell marker. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abu
Biotium Connexin 32 (Gap Junction Protein) (GJB1/1753), CF568 conjugate, 0.1mg/mL
This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helicaldomains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S a
Biotium PMEPA1 / TMEPAI (Tumor Suppressor Oncoprotein)(PMEPA1/2697), CF568 conjugate, 0.1mg/mL
PMEPA1 (prostate transmembrane protein, androgen induced 1 is a 287 amino acid single-pass membrane protein that contains WW-binding motifs and localizes to the cell membrane. Expressed at high levels in prostate, kidney and ovary, PMEPA1 interacts with NEDD4 and may play a role in regulating AR (androgen receptor) levels, specifically in prostate cells. Down regulation of PMEPA1 is observed in prostate tumors, suggesting that PMEPA1 may exhibit activity as a tumor suppressor. Overexpression of this protein may play a role in multiple types of cancer.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium CD73 (Immuno-Oncology Target)(NT5E/2646), CF568 conjugate, 0.1mg/mL
CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in Mouse tumor models, and could enhance t
Biotium Melanoma Antigen Family A, 4 / MAGEA4(CPTC-MAGEA4-1), 1mg/mL
The melanoma-associated antigen (MAGE) family consists of a number of antigens recognized by cytotoxic T lymphocytes. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types, but not in normal tissues, with the exception of testis and placenta. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium Sulfotransferase family 1E, estrogen-preferring, member 1(CPTC-SULT1E1-1), 0.2mg/mL
The soluble sulfotransferases contribute to the elimination of xenobiotics, the activation of procarcinogens and the regulation of hormones by catalyzing the sulfate conjugation of these substances. SULT1E1, also known as STE, is a 294 amino acid member of the SULT1 family. Localized to the cytoplasm and expressed in intestine, liver and kidney, SULT1E1 exists as a homodimer that is thought to control estrogen receptor (ER) levels by sulfurylating free estradiol. Defects in the gene encoding SULT1E1 are associated with an increased risk for endometrial cancer, suggesting a role for SULT1E1 in tumorigenesis. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than o
Biotium GPX4 / MCSP(LHM 2), 1mg/mL
The antioxidant enzyme glutathione peroxidase 4 (GPx4) catalyzes the reduction of peroxides through oxidation of cellular glutathione.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (KRTL/1577R), CF740 conjugate, 0.1mg/mL
This MAb recognizes the 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (VWF/1465), CF740 conjugate, 0.1mg/mL
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and C
Biotium CD257 / BAFF / TNFSF13B (C257/1638), 0.2mg/mL
BAFF/CD257 is a type II transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. It is proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homo-trimers. The predicted molecular weight of a BAFF monomer is approximately 31 kDa. It is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells and is upregulated by IFN-gamma and down-regulated by PMA/ionomycin treatment. BAFF stimulates B and T cell immunity and regulates humoral immunity by binding TACI and BCMA receptors; BAFF prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because