DNA Reagents
Aldevron gWiz™ GFP RG 5mg
5mg dispense of gWiz GFP plasmid. The gWiz plasmids are designed for the highest level of gene expression in a wide range of mammalian cells and tissues. They contain a proprietary modified promoter, followed by intron A from the CMV immediate early gene, and a high-efficiency artificial transcription terminator. gWiz expression vectors are constructed using a plasmid backbone that has been extensively modified to achieve enhanced levels of transgene expression in mammalian cells.hese ready-to-use plasmids are endotoxin-free and can be applied directly to most in vitro and in vivo applications. Each gWiz plasmid is delivered in the buffer of your choice with delivery time of approximately 24 to 48 hours.
This product is subject to the following: Limited Use License
Sigma Aldrich Fine Chemicals Biosciences pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution | 93384-17-9 | MFCD00146650 | 1UN
pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution | 93384-17-9 | MFCD00146650 | 1UN
Aldevron pALD-AAV2 10mg
10mg dispense of AAV2 RepCap plasmid for use in an AAV triple transfection.
This product is subject to the following: Limited Use License
Revvity Health Sciences Inc NEXTFLEX Cas9-gRNA rRNA Depletion Enzyme removes rRNA from human, mouse, and rat RNA-seq libraries using ready-to-use CRISPR-Cas9 depletion.
The NEXTFLEX™ Cas9-gRNA rRNA Depletion Enzyme (HMR) powered by Jumpcode DepleteX™ technology, is a standalone, workflow-agnostic depletion enzyme that removes ribosomal RNA sequences from human, mouse, or rat libraries. You can drop it into any bulk or single cell RNA-seq workflow, increasing usable reads for informative transcripts and improving coverage across the transcriptome.
NEXTFLEX Cas9-gRNA rRNA Depletion Enzyme is provided at 5.6 μM and includes 435 unique guide RNAs targeting nuclear 5S, 5.8S, 18S, 28S, 45S and mitochondrial 12S and 16S rRNA loci. It requires a simple one-hour incubation making integration straightforward in pre- or post-PCR cleanup steps depending on your workflow. Designed to be highly cost-competitive and universally compatible, it delivers robust rRNA depletion without locking you into a specific library prep kit.
Aldevron gWiz™ Blank RG 10mg
10mg dispense of gWiz plasmid. The gWiz plasmids are designed for the highest level of gene expression in a wide range of mammalian cells and tissues. They contain a proprietary modified promoter, followed by intron A from the CMV immediate early gene, and a high-efficiency artificial transcription terminator. gWiz expression vectors are constructed using a plasmid backbone that has been extensively modified to achieve enhanced levels of transgene expression in mammalian cells.hese ready-to-use plasmids are endotoxin-free and can be applied directly to most in vitro and in vivo applications. Each gWiz plasmid is delivered in the buffer of your choice with delivery time of approximately 24 to 48 hours.
This product is subject to the following: Limited Use License
ABclonal Technology AFTSpin Plasmid Mini Kit
The kit is capable of lysing cells using a modified SDS-alkaline lysis method. The optimized lysate allows the silica-based membrane in the centrifugal adsorption column to selectively bind the plasmid DNA in the solution under the condition of high salt and low pH value and then removes impurities and other bacterial components by deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes the pure plasmid DNA from the silica-based membrane.
In general, a high-copy plasmid is inoculated from a single colony in 1.5-4.5 mL of LB medium supplemented with appropriate antibiotics. After overnight culture for 12-16 hours, up to 20 ug of pure plasmid can be extracted.
Aldevron Custom Gigaprep, AmpR, Water
Take the burden of prepping plasmids off your hands by sending your plasmid preps to Aldevron. In as fast as 72 hours from receipt of your starting material, we will make a 1L batch scale of your plasmid and deliver all plasmid purified from that scale, typically yielding approximately 10mg* delivered. You will receive your Ampicillin-resistant plasmid delivered in water with UV Spec QC.
To initiate this project, you can add the quantity of plasmids required to your Fisher Cart.
REQUIRED:
Download this submission form and send the completed form to order@aldevron.com. We’ll be able to get your project started upon receipt of your starting materials as described in the form. Starting Material Submission Form
*Note: plasmid yield not guaranteed, yield estimate provided for information only
Takara Bio Chaperone Plasmid Set, 1 Set
Consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function as a "chaperone team" to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cmr gene, which allows use with E. coli expression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of an araB or Pzt-1 (tet) promoter. Therefore, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac). These plasmids also contain the necessary regulator (araC or tetr) for each promoter. 1 Set
Revvity Health Sciences Inc Tag-lite pSNAP-GLP1 Glucagon Receptor Plasmid, 10 µg
Tag-lite pSNAP-GLP1 Glucagon Receptor Plasmid, 10 µg
Revvity Health Sciences Inc Tag-lite pSNAP-GIPR Glucagon Receptor Plasmid, 10 µg
Tag-lite pSNAP-GIPR Glucagon Receptor Plasmid, 10 µg
Sigma Aldrich Fine Chemicals Biosciences pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution | 93384-17-9 | MFCD00146650 | 5UN
pBR322 Plasmid DNA from E. coli RRI buffered aqueous solution | 93384-17-9 | MFCD00146650 | 5UN
Aldevron pALD-X80 10mg
10mg dispense of pALD-X80 helper plasmid. 18.9kb, Kanamycin resistant, license and royalty free from research through commercial applications. pALD-X80 has a history of use in clinical trials, and has a Drug Master File on file with the US FDA.
This product is subject to the following: Limited Use License
Aldevron pALD-ITR-GFP 10mg
10mg dispense of GFP reporter version of the pALD-AAV system suitable for testing and optimization.
This product is subject to the following: Limited Use License
Aldevron Custom Gigaprep, AmpR, Tris
Take the burden of prepping plasmids off your hands by sending your plasmid preps to Aldevron. In as fast as 72 hours from receipt of your starting material, we will make a 1L batch scale of your plasmid and deliver all plasmid purified from that scale, typically yielding approximately 10mg* delivered. You will receive your Ampicillin-resistant plasmid delivered in Tris buffer (10mM Tris, pH 8.0) with UV Spec QC.
To initiate this project, you can add the quantity of plasmids required to your Fisher Cart.
REQUIRED:
Download this submission form and send the completed form to order@aldevron.com. We’ll be able to get your project started upon receipt of your starting materials as described in the form. Starting Material Submission Form
*Note: plasmid yield not guaranteed, yield estimate provided for information only
Revvity Health Sciences Inc Tag-lite pSNAP-OX2 Orexin Receptor Plasmid, 10 µg
Tag-lite pSNAP-OX2 Orexin Receptor Plasmid, 10 µg