Chemical reagents, kits, columns, and other products used for DNA synthesis, and to subsequently run purification columns; includes genetic assembly systems.
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A TAAR1 agonist increases intracellular cAMP levels in cells expressing TAAR1 (EC50 0.14 UM) an agonist of 5-HT1D and 5-HT1A receptors decreases cAMP levels in cells expressing 5-HT1D and 5-HT1A receptors (EC50s 0.262 and 2.3 UM respectively) selective for these receptors over the dopamine D2L receptor (EC50s 10.44 and 8.02 UM in cAMP and arrestin recruitment assays respectively) as well as a2A- and a2B- adrenergic receptors and 5-HT1B 5-HT2A 5-HT2C and 5-HT7 receptors (EC50s 6.7-10 UM in functional assays) reduces PCP-induced hyperactivity in mice at 0.3 1 and 3 mg/kg increases prepulse inhibition of the acoustic startle response in mice at 3 10 and 30 mg/kg
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DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
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Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3 5 proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100C.
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RecJf is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxy-nucleotide monophosphates from DNA in the 5' to 3' direction. RecJf is a recombinant fusion protein of RecJ and maltose binding protein (MBP). It has the same enzymatic properties as wild-type RecJ. Fusion to MBP enhances RecJf solubility. When DNA containing a 22 base 5' extension is used as a substrate for RecJf, the resulting products are a mixture of DNA fragments that have blunt-ends, 5' extensions (1-5 nucleotides) and recessed 5' ends (1-8 nucleotides). RecJf does not require a 5' phosphate.
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