Transfection Reagents
Lonza Walkersville SF Cell Line 4D-Nucleofector LV Kit XL, Catalog Number V4LC-2002, Reaction Volume Up to 20 mL, Kit Size 5 reactions, Processing Time Less than 10 min, Compatible System 4D-Nucleofector LV Unit
The Lonza SF Cell Line 4D-Nucleofector LV Kit XL is specifically designed for efficient, large-scale transfection of hard-to-transfect cell lines using the 4D-Nucleofector LV Unit. This closed-system nucleofection kit modifies up to 1 x 10^9 cells effectively, retaining cell viability. It allows variable reaction volumes and rapid processing, making it ideal for transient protein production and generating modified cell lines for various assays.
- Compatible system: 4D-Nucleofector LV Unit
- Reaction volume: Up to 20 mL
- Cell numbers supported: Up to 1 x 10^9 cells
- Processing time: Less than 10 minutes
- Kit size: 5 reactions
- Optimal cell lines: HEK293 suspension clones, CHO-S suspension clones, K-562, U-937
- Applications: Large scale transient protein production, generation of modified cell lines
- Key benefits: High transfection efficiencies, maintained cell functionality
- Storage conditions: 2 to 8 °C
LGC GENOMICS LLC (Lucigen) TransforMax EC100D pir-116 Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Takara Bio XFECTA PROTEIN TRANS RGNT 30PK
Xfect Protein is a novel, peptide-based transfection reagent that efficiently delivers a large amount of biologically active protein directly into live cells, with low cytotoxicity.
SignaGen Laboratories GENJET DNA REAGENT PAC 1ML
GenJet DNA In Vitro Tranfection Reagent for PAC was pre-optimized for most efficiently transfecting PAC cells.
Apexbio Technology LLC PPACK Dihydrochloride 142036-63-3 5mg
PPACK Dihydrochloride is a small-molecule inhibitor targeting human -thrombin It is designed to inhibit thrombin activity thereby blocking downstream platelet aggregation and coagulation pathways PPACK Dihydrochloride exerts its biological activity primarily through selective irreversible inhibition of thrombin by covalently occupying the thrombin active-site serine residue and forming a stable tetrahedral intermediate with the catalytic His57 residue Based on these pharmacological properties PPACK Dihydrochloride holds research potential in biomedical studies as an anticoagulant reagent and for investigating thrombin-mediated coagulation and platelet activation mechanisms
ABclonal Technology DLDH/DLD Rabbit pAb
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO GREEN TRANSF IND 50NMOL
siGLO Green Transfection Indicator, 50nmol
SignaGen Laboratories GENJET DNA REAGENT 3LL 1ML
GenJet DNA In Vitro Tranfection Reagent for 3LL was pre-optimized for most efficiently transfecting 3LL cells.
SignaGen Laboratories GENJET DNA REAGENT B16 1ML
GenJet DNA In Vitro Tranfection Reagent for B16 was pre-optimized for most efficiently transfecting B16 cells.
STEMCELL Technologies Resazurin (Sodium Salt), 5 g
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media and further differentiated into cells of all three germ layers (Figure 7).