Transfection Reagents
Eurofins Discoverx KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B), 2 vials x 0.5 (Adherent), 2 vials x 1.0 (Suspension). (97-0008)
The KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-adherent-c-97-0008.
Sigma Aldrich Fine Chemicals Biosciences DOTAP Liposomal Transfection Reagent | 144189-73-1 | MFCD16660441 | 5 x 400 uL
DOTAP Liposomal Transfection Reagent | Purity: 99% | Mol Wt: 774.19 | 144189-73-1 | MFCD16660441 | 5 x 400 uL
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
New England Biolabs, Inc. NEB® 10-beta Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Expression Systems BestBac 2.0 Δ v-cath/chiA Baculovirus Cotransfection Kit
Expression Systems popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.
Unmatched productivity and stability for BEVS
Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/mL of recombinant baculovirus in P1 stock
Generates P1 stock as quickly as Bac-to-Bac and other systems
Each BestBac vial contains enough material for a minimum of five cotransfections
Suitable for other expression systems including BacMam™
Contains everything you need to perform cotransfections with your transfer vector. Included in the kit are:
• BestBac DNA
• pVL1393-lacZ control vector
• Transfection medium
Expres2 TR transfection reagent
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 4 TRANSFECTION REAG
NC1381290 DHARMAFECT 4 TRANSFECTION REAG
Genesee Scientific Corporation TRANSFECTION REAGENT 0.1ML
0.1ml Reagent with 10ml Buffer0.1ml/UnitBrand: Polyplus-transfectionBest-in-Class DNA Transfection ReagentBased on Novel Cationic NanotechnologyReach Maximal Gene Expression in Hard-to-Transfect CellsExcellent Cell Viability & MorphologyHighly Efficient: Requires Minimal Reagent & DNAReady-to-Use ProtocolCost Effective
System Biosciences LLC TRANSFECTION-READY CAS9 MRNA
Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version) 20 ug
Lonza Walkersville Human Stem Cell Nucleofector Kit 1
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 1 is one of our two kits suited for efficient transfection of human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 1, 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT SET OF 4 TRANSFECTI
Dharmafect Set of 4 transfection reagent
Cell Biolabs Inc CytoSelect™ 96-Well Cell Transformation Assay Kit (Cell Recovery Compatible, Colorimetric), 96 assays
The traditional soft agar colony formation assay monitors anchorage-independent cell growth by measuring proliferation in a semisolid culture media in tissue culture dishes after 3-4 weeks, followed by manual counting of colonies. This method is cumbersome and not amenable to high throughput, and the manual counting of colonies is subjective because with varying colony sizes, it’s difficult to determine meaningful results. The CytoSelect™ 96-well Cell Transformation Assay (Cell Recovery Compatible) eliminates subjective manual counting of colonies with a much shorter assay time. Cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the provided colorimetric dye in a standard plate reader, making it a more quantitative and higher-throughput method. Additionally, the proprietary medium makes it possible to recover viable and intact cells for further downstream analysis. Each kit is sufficient for 96 tests in a microtiter plate.