Transfection Reagents
Lonza Walkersville Mouse Neural Stem Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary mouse neural stem cells (NSCs).The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Neural Stem Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Zymo Research Corporation Rattler™ Plating Beads (230 g) (5 Bottles) ^
The sterile glass beads are simply poured onto solid plated medium together with a liquid cell suspension, and the mixture is shaken to distribute the cells evenly over the medium's surface. This allows for numerous plates to be processed quickly and efficiently. Pour the Rattler Beads onto a series of plates, stack, and shake simultaneously in a side-to-side motion. The beads can be easily removed following inversion of the plates and pouring off from the plate lids. Using the Rattler Plating Beads is simple, easy, and saves you time. The beads come sterile in polycarbonate bottles and can be reused following cleaning and washing.Rattler™ Plating Beads Fast & Easy-To-Use: Simply pour beads onto the plate, and shake to quickly spread cells evenly over the entire surface. Ideal for processing multiple plates simultaneously. No Flaming Required: 4.5 mm glass plating beads are provided sterile in polycarbonate bottles. Reusable: Just clean and autoclave after use.
Andwin Scientific TRANSFECTION REAGENT + BUFFER
The jetPRIME™ DNA and siRNA Transfection Reagent is made to guarantee high DNA transfection efficiency and excellent gene silencing. This product is very cost-effective as it uses less reagent and DNA and provides more gentle transfections in comparison to other competitors. It can be used with no difficulty and with serum and antibiotics. This can produce 188 reactions in 6-well plates and has a buffer volume of 60 mL. It is sold individually.
Sigma Aldrich Fine Chemicals Biosciences Hexadimethrine bromide | 28728-55-4 | MFCD00133397 | 100 g
Hexadimethrine bromide | Purity: ≥94% | Mol Wt: 1122.61 | 28728-55-4 | MFCD00133397 | 100 g
Active Motif Chariot™
Chariot is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the need for fixing. This makes Chariot an ideal tool for functional studies, including delivering inhibitory proteins, labeling organelles, screening peptide libraries and studying protein half-lives & transient complementation.
Sigma Aldrich Fine Chemicals Biosciences Hexadimethrine bromide | 28728-55-4 | MFCD00133397 | 50g
Hexadimethrine bromide | 94% | 28728-55-4 | MFCD00133397 | 50g
ABclonal Technology DLDH/DLD Rabbit pAb
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Biolegend Dry Human AIM Assay Cocktail K
Dry Human AIM Assay Cocktail Kit; Reactivity: Human; Apps: FC; Size: 5 tests
Lonza Walkersville Human Stem Cell Nucleofector Starter Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit. The Human Stem Cell Nucleofector Starter Kit is used to determine which one of our two Nucleofector Kits is best suited for transfecting a specific primary human stem cell clone in Nucleofector 2b Device. It is suitable for 18 optimization reactions.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format