Transfection Reagents
Lonza Walkersville P3 Primary Cell 4D-Nucleofector LV Kit XL
The P3 Primary Cell 4D-Nucleofector LV Kit XL* is suited for larger scale transfection of primary cells in the LV Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the LV Nucleocuvette Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.Applications:For large scale transfection of up to 1 x 10⁹ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 1,1 x LV Nucleocuvette Cartridge2 x 4D-Nucleofector LV Reservoir
HORIZON DISCOVERY BIOSCIENCES LIMITED DharmaFECT 1 Transfection Reagent | 5x1.5ml
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments. DharmaFECT 1 has been validated in over 35 cell types.
Lonza Walkersville Cell Line Nucleofector Kit T
The Cell Line Nucleofector Kit T is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A431A549Caco-2CHO-K1D1 ORL UVAHT-1080NALM-6 (DSMZ)NRKP815RBL-2H3T84TF-1U-87 MGThe respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution T0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
BIOLINK LABORATORIES LLC FECTOPRO TRANSFEC REAGENT 10ML
+10mL FectoPRO boosterReagent:FectroPRO®Molecule Delivered:DNAApplications:BioproductionCell Types:Suspension CHO, HEK-293, and derivativesNumber of Transfections:1mL of FectoPRO® is sufficient to transfect 800mL to more than 1L of cell cultureStorage:5°C ± 3°C, stable for 1 to 2 years when stored appropriatelyProvided with:FectoPRO® Booster
HORIZON DISCOVERY BIOSCIENCES LIMITED HUMAN MTAP / DLD-1 CELL LINE
NC2674184 HUMAN MTAP / DLD-1 CELL LINE
