Molecular Biology Reagents and Kits
System Biosciences LLC pGreenFire 2.0 NFκB Reporter Lentivector & ViruspGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro) = >2 x 10^6 IFUs
Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter PLASMID (red firefly luciferase & GFP), which is engineered for reliable stable cell line generation. SBI has upgraded our popular pGreenFire signaling pathway reporter lentivectors with a design that leads to more reliable generation of stable cell lines. We’ve also swapped in the red firefly luciferase reporter (rFLuc), which opens up the possibility of performing a dual-spectral luciferase assay and delivers greater sensitivity for in vivo applications than conventional luciferase.With the pGreenFire 2.0 NFκB Reporter Lentivector & Virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro), the core reporter functionality is similar to the original pGreenFire lentivector—NF-κB transcriptional response elements (TREs) are placed upstream of a minimal CMV promoter (mCMV) which together drive co-expression of rFLuc and GFP in response to NF-κB activity.
