Molecular Biology Reagents and Kits
Zymo Research Corporation RNA Clean & Concentrator MagBead (supplied with DNaseI set) (96 preps)
The procedure is simple: Add binding buffer and MagBinding Beads to your sample, then wash and elute ultra-pure RNA. The unique singlebuffer system and magnetic bead technology can be used on an automated platform (or manually) for high-throughput processing.
Zymo Research Corporation M-Binding Buffer, 250 ml
Used for purification of bisulfite converted DNA on a Zymo-spin column in the EZ DNA Methylation, EZ DNA Methylation - Gold, EZ DNA Methylation - Direct kits, and EZ DNA Methylation - Lightning kits.
Zymo Research Corporation ZymoBIOMICS-96 Magbead DNA Kit (lysis Matrix Not Included) (2 x 96 preps)
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Zymo Research Corporation ZymoPURE™ P3 (Yellow) (100 ml)
ZymoPURE™ P3 (Yellow) (100 ml). For use in ZymoPURE™ Plasmid Purification kits. Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation EZ DNA Methlyation-Lightning Kit (10 rxns.)
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process - simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 l. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
New England Biolabs, Inc. G(5')ppp(5')A RNA Cap Structure Analog – 1 umol
The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5' terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas. Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5' )ppp(5' )G.
Quality Biological Inc Sodium Chloride 5 M, 10 Liters
Sodium Chloride, 5M is suitable for molecular biology, protein chemistry and biochemical applications. ¿ 5 M concentration ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Sodium Chloride, 5 M is free of detectable endonucleases, ribonucleases and nicking activity according to the current quality control procedures. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 41105514 ¿ ¿ Max Shelf Life: 36 months
Vector Laboratories Mouse On Mouse (M.O.M.) ImmPRESS HRP (Peroxidase) Polymer Kit
Vector M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue. This problem can be essentially eliminated by using Vector M.O.M. technology. The result is clear, crisp specific staining of the antigens of interest. This Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step M.O.M. ImmPRESS Peroxidase Polymer reagent. The highly active peroxidase micropolymers of the ImmPRESS Detection System provide outstanding sensitivity, limit steric interference, and enhance accessibility. Contains sufficient reagents to stain approximately 150 mouse sections. Includes 0.6 ml M.O.M. Mouse Ig Blocking Reagent (concentrate), 30 ml R.T.U. 2.5% Normal Horse Serum, and 15 ml R.T.U. M.O.M. ImmPRESS Anti-Mouse Ig Reagent.