Molecular Biology Reagents and Kits
Vector Biolabs Cyclin-dependent kinase 7 Aden
CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-Cas9)
IBA LifeSciences StrGate Acptr Vctr pASG-IBA2
The pASG-IBA2 vector is designed for expression of recombinant proteins with a C-terminal Strep-tagII in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. Expression of the protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via Entry Vector is possible.