DNA Vectors
Invivogen Human kappa, pFUSE2ss-CLIg-hK, 20 ug
pFUSE2ss-CLIg-hK additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
BAYOU BIOLABS M13mp18 Single-Stranded Phage DNA, 500 μg, 1.00 μg/μL, Highly purified, Minimal contamination, 10 mM Tris-Cl, 1 mM EDTA, pH 8.0, Store at -20 °C
This product is a highly purified M13mp18 single-stranded phage DNA that is essential for various molecular biology applications. It is engineered for efficient production of single-stranded DNA templates, making it suitable for dideoxy DNA sequencing, site-directed mutagenesis, and cloning. The product features a multiple cloning site within the lacZ' region, facilitating blue/white colony screening for recombinant selection, and is also suitable for advanced applications such as phage display and DNA origami.
- Type: Single-stranded phage DNA
- Purity: Highly purified, free of contaminants
- Quantity supplied: 500 μg
- Concentration: 1.00 μg/μL
- Storage temperature: -20 °C or below
- Quality control: Confirmed by electrophoresis
- Applications: DNA sequencing, cloning, site-directed mutagenesis
- Vector length: 7249 bp
Vector Biolabs IFN-gamma; Adenovirus (Ad-CMV-IFN-gamma)
Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immuno-regulatory and pro-inflammatory activities and is thus important in host defense mechanisms. IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites.
Vector Biolabs Caspase 9 (dn) Adenovirus (Ad-CMV-Caspase 9 (DN))
This adenovirus contains a Caspase 9 (C287A mutant).
Vector Biolabs AAV-Cre-GFP (AAV serotype 6) AAV (AAV6-Cre-GFP)
This AAV serotype 6 virus (with Capsid from AAV6 and ITR from AAV2) expresses Cre Recombinase (tagged with nuclear localization sequence) and eGFP marker. Each gene expression is driven by its own CMV promoter.Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
Vector Biolabs Interleukin-1 receptor-associated kinase 3 Adenovirus (Ad-IRAK3)
Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL-1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway. 100kDa IRAK-1 is implicated as an upstream mediator of NFkB activation. IRAK-2 is a proximal mediator of IL-1, a component of the IL-1R signaling complex and is required for IL-1R-induced NF?B activation. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells.
Vector Biolabs C-reactive protein, pentraxin-related Adenovirus (Ad-CRP)
Inflammation results in a rapid increase in blood levels of acute phase proteins synthesized by hepatocytes in response to cytokines. Pentraxins, which include C reactive protein (CRP) and serum amyloid P component (SAP), are prototypic acute phase proteins. CRP and SAP are produced by liver epithelial cells and are characterized by a cyclic pentameric structure and calcium-dependent ligand binding. Human serum amyloid A (SAA) is also a major acute-phase protein and precursor to amyloid A protein (AA), which is the major constituent of the fibril deposits of reactive amyloidosis.
Vector Biolabs Akt1 (dn) Adenovirus (Ad-CMV-Akt1 (dn))
An HA tag was fused in frame to the N termini of the coding sequence of mouse Akt1(T308A, S473A) mutant, which is also known as Akt1(AA). Akt1/PKB is activated in response to many extracellular signals and play key roles in regulating diverse cellular processes, including cell proliferation, differentiation, apoptosis, angiogenesis and glycogen synthesis etc. Akt1 is activated by the phosphorylation of the T loop of their kinase domain by PDK1 and by phosphorylation of a residue located C-terminal to the kinase domain in the hydrophobic motif. The Akt family of kinases includes Akt1, Akt2 and Akt3.
Vector Biolabs Interferon regulatory factor 2 Adenovirus (Ad-IRF2)
IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.
Vector Biolabs Histone deacetylase 8 Adenovirus (Ad-HDAC8)
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical process in transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the N-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility of transcription factors to DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Mammalian nuclear histone acetylases include GCN5, PCAF (p300/CBP-associated factor), HAT1, MOF, HBO, MOZ, MORF and TIP60.1.The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.
Vector Biolabs Baculoviral IAP repeat-containing 4 Adenovirus (Ad-BIRC4/XIAP)
The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Similar to API1, BIRC4 inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.
