DNA Vectors
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
Sigma Aldrich Fine Chemicals Biosciences CRISPR LacZ Positive Contr1EA
Recent publications using CRISPR/Cas9-mediated recombineering in E. coli tout editing efficiencies near 100% making CRISPR/Cas9-mediated recombineering the most powerful bacterial genome engineering method to date. In addition Cas9-mediated recombineering overcomes the dependence on a second recombination step avoids the creation of destabilizing scar sites can be used in multiplexing and is less time-consuming than previous protocols.Here we present a novel dual-vector CRISPR/Cas-mediated»-Red system for improved recombineering in E. coli. Our system is shown to facilitate homology-directed repair of DSBs created by Cas9 endonuclease enabling genetic alterations through chromosomal integration of a donor DNA.This plasmid is to be used in combination with the Cas9 Lambda Red homologous recombination plasmid for E. coli (CAS9BAC1P) as the positive control for your custom gene editing experiment. The custom gRNA (CRISPRBACD) can be designed and ordered through https//www.sigmaald
Gentarget PSA-GFP PURO LENTIVIRUS
Pre-made lentivirus express GFP fluorescent reporter under a native human PSA promoter for selective expression in Prostate tissue or Cells.This lentivirus also contain the Puromycin selection marker under the constitutive RSV promoter for antibiotic selection. Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS.
BIOHIPPO AAV-H1-shRNA(scramble)-CMV-EGFP-SV40-polyA
Recombinant adeno associated virus expressing shRNA (scramble) fused to EGFP under the H1 CMV promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.