DNA Vectors
System Biosciences LLC PGREENFIRE1-LXRE IN LXRA HEPG2 CELL LINE
Study cholesterol homeostasis by co-expressing dscGFP and luciferase in response to Liver X Receptor (LXR) activity at the LXRα promoter in HepG2 Cells. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGreenFire1-LXRE-in-LXRα Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from the Liver X Receptor (LXR) response elements (LXREs) and neighboring regions in the LXRα promoter paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of the LXRE/LXRα transcriptional elements, drives expression of dscGFP and luciferase in response to LXR activity. The result is the ability to quantitatively measure LXR activity at the LXRα promoter by fluorescence and luciferase activity.
